Rabbit anti-Human, Mouse ACOT12 Polyclonal Antibody | anti-ACOT12 antibody

ACOT12 (R315) Polyclonal Antibody

Cat. Num. AAA3013907

• Gene Names: ACOT12; Cach; THEAL; CACH-1; STARD15
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ACOT12; Polyclonal Antibody; ACOT12 (R315) Polyclonal Antibody; Acyl-coenzyme A thioesterase 12; Acyl-CoA thioesterase 12; Acyl-CoA thioester hydrolase 12; Cytoplasmic acetyl-CoA hydrolase 1; CACH-1; hCACH-1; START domain-containing protein 15; StARD15; CACH; CACH1; STARD15; anti-ACOT12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ACOT12 (R315) polyclonal antibody detects endogenous levels of ACOT12 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 555

• Applicable Applications for anti-ACOT12 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 285-345 of Human ACOT12.
• Preparation and Storage: Store at 4°C short term.; Aliquot and store at -20°C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ACOT12 (R315) polyclonal antibody at 1:1000 dilutionLane1:AML-12 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug))

Related Product Information for anti-ACOT12 antibody

Abstract Acetyl-CoA plays a fundamental role in cell signaling and metabolic pathways, with its cellular levels tightly controlled through reciprocal regulation of enzymes that mediate its synthesis and catabolism. ACOT12, the primary acetyl-CoA thioesterase in the liver of human, mouse, and rat, is responsible for cleavage of the thioester bond within acetyl-CoA, producing acetate and coenzyme A for a range of cellular processes. The enzyme is regulated by ADP and ATP, which is believed to be mediated through the ligand-induced oligomerization of the thioesterase domains, whereby ATP induces active dimers and tetramers, whereas apo- and ADP-bound ACOT12 are monomeric and inactive.

NCBI and Uniprot Product Information

• NCBI GI #: 18640736
• NCBI GeneID: 134526
• NCBI Accession #: NP_570123.1
• NCBI GenBank Nucleotide #: NP_570123.1
• UniProt Accession #: Q8WYK0
• Molecular Weight: ~ 62kDa
• NCBI Official Full Name: acetyl-coenzyme A thioesterase
• NCBI Official Synonym Full Names: acyl-CoA thioesterase 12
• NCBI Official Symbol: ACOT12
• NCBI Official Synonym Symbols: Cach; THEAL; CACH-1; STARD15
• NCBI Protein Information: acetyl-coenzyme A thioesterase
• UniProt Protein Name: Acyl-coenzyme A thioesterase 12
• Protein Family: Acyl-coenzyme A thioesterase
• UniProt Gene Name: ACOT12
• UniProt Synonym Gene Names: CACH; CACH1; STARD15; Acyl-CoA thioesterase 12; CACH-1; hCACH-1; StARD15
• UniProt Entry Name: ACO12_HUMAN

Uniprot Description

ACOT12: Hydrolyzes acetyl-CoA to acetate and CoA.

Protein type: EC 3.1.2.1; Hydrolase; Carbohydrate Metabolism - pyruvate

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: cytosol

Molecular Function: acetyl-CoA hydrolase activity; lipid binding; ATP binding

Biological Process: acyl-CoA metabolic process; acetyl-CoA metabolic process; pyruvate metabolic process; fatty acid metabolic process