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Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Cerebellum Tissue; Lane3: Mouse Lung Tissue.)
Rabbit anti-Human Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A) Polyclonal Antibody | anti-ANP32A antibody
Polyclonal Antibody to Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A)
Gene Names
ANP32A; LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Acidic Nuclear Phosphoprotein 32 Family; Member A (ANP32A); Polyclonal Antibody; Polyclonal Antibody to Acidic Nuclear Phosphoprotein 32 Family; anti-ANP32A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ANP32A. It has been selected for its ability to recognize ANP32A in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGS-EMGRRIHLE LRNRTPSDVK ELVLDNSRSN EGKLEGLTDE FEELEFLSTI NVGLTSIANL PKLNKLKKLE LSDNRVSGGL EVLAEKCPNL THLNLSGNKI KDLSTIEPLK KLENLKSLDL FNCEVTNLND YRENVFKLLP QLTYLDGYDR DDKEAPDSDA EGYVEGLDDE EEDEDEEEYD EDAQVVEDEE DEDEEEEGEE EDVSGEEEED EEGYNDGEVD DEEDEEELGE EERGQKRKRE PEDEGEDDD
MGHHHHHHSGS-EMGRRIHLE LRNRTPSDVK ELVLDNSRSN EGKLEGLTDE FEELEFLSTI NVGLTSIANL PKLNKLKKLE LSDNRVSGGL EVLAEKCPNL THLNLSGNKI KDLSTIEPLK KLENLKSLDL FNCEVTNLND YRENVFKLLP QLTYLDGYDR DDKEAPDSDA EGYVEGLDDE EEDEDEEEYD EDAQVVEDEE DEDEEEEGEE EDVSGEEEED EEGYNDGEVD DEEDEEELGE EERGQKRKRE PEDEGEDDD
Sequence Length
249
Applicable Applications for anti-ANP32A antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant ANP32A (Glu2~Asp249) expressed in E.coli.
Cross Reactivity
Human, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2081536
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Cerebellum Tissue; Lane3: Mouse Lung Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Cerebellum Tissue; Lane3: Mouse Lung Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ANP32A, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ANP32A, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: ANP32A knockout Hela cell lysate; Predicted MW: 29kd Observed MW: 35kd Primary Ab: 1ug/ml Rabbit Anti-Human ANP32A Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: ANP32A knockout Hela cell lysate; Predicted MW: 29kd Observed MW: 35kd Primary Ab: 1ug/ml Rabbit Anti-Human ANP32A Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Product Categories/Family for anti-ANP32A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,585 Da
NCBI Official Full Name
acidic leucine-rich nuclear phosphoprotein 32 family member A
NCBI Official Synonym Full Names
acidic nuclear phosphoprotein 32 family member A
NCBI Official Symbol
ANP32A
NCBI Official Synonym Symbols
LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
NCBI Protein Information
acidic leucine-rich nuclear phosphoprotein 32 family member A
UniProt Protein Name
Acidic leucine-rich nuclear phosphoprotein 32 family member A
Protein Family
UniProt Gene Name
ANP32A
UniProt Synonym Gene Names
C15orf1; LANP; MAPM; PHAP1; pp32; LANP; PHAPI
Uniprot Description
ANP32A: Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase- independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1- mediated transcriptional repression. Belongs to the ANP32 family.
Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 15q23
Cellular Component: cytoplasm; endoplasmic reticulum; nucleoplasm; nucleus; perinuclear region of cytoplasm
Molecular Function: histone binding; protein binding; RNA binding
Biological Process: histone exchange; nucleocytoplasmic transport; nucleosome assembly; regulation of apoptosis; regulation of mRNA stability; regulation of transcription, DNA-templated; transcription, DNA-dependent
Research Articles on ANP32A
Similar Products
Product Notes
The ANP32A anp32a (Catalog #AAA2003917) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ANP32A anp32a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG S-EMGRRIHL E LRNRTPSDVK ELVLDNSRSN EGKLEGLTDE FEELEFLSTI NVGLTSIANL PKLNKLKKLE LSDNRVSGGL EVLAEKCPNL THLNLSGNKI KDLSTIEPLK KLENLKSLDL FNCEVTNLND YRENVFKLLP QLTYLDGYDR DDKEAPDSDA EGYVEGLDDE EEDEDEEEYD EDAQVVEDEE DEDEEEEGEE EDVSGEEEED EEGYNDGEVD DEEDEEELGE EERGQKRKRE PEDEGEDDD. It is sometimes possible for the material contained within the vial of "Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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