Rabbit anti-Human, Mouse ACE Polyclonal Antibody | anti-ACE antibody

ACE Antibody

Cat. Num. AAA9408389

• Gene Names: ACE; DCP; ICH; ACE1; DCP1; CD143; MVCD3
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antigen affinity purification.
• Synonyms: ACE; Polyclonal Antibody; ACE Antibody; DCP; ICH; ACE1; DCP1; CD143; MVCD3; anti-ACE antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total ACE protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 1.2 mg/ml (varies by lot)
• Sequence Length: 1306

• Applicable Applications for anti-ACE antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:1000-1:5000
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to a region derived from internal residues of human Angiotensin-converting enzyme
• Target Name: ACE
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 6%SDS-PAGE Lysates (from left to right): Mouse kidney tissue Amount of lysate: 40ug per lane Primary antibody: 1/1000 dilution Secondary antibody dilution: 1/8000 Exposure time: 30 seconds)

Related Product Information for anti-ACE antibody

This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active.

Product Categories/Family for anti-ACE antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4503273
• NCBI GeneID: 1636
• NCBI Accession #: NP_000780.1
• NCBI GenBank Nucleotide #: NM_000789.3
• UniProt Accession #: P12821; P22966; Q53YX9; Q59GY8; Q7M4L4; B0LPF0; B4DXI3; E7EU16
• Molecular Weight: 78,694 Da
• NCBI Official Full Name: angiotensin-converting enzyme isoform 1
• NCBI Official Synonym Full Names: angiotensin I converting enzyme
• NCBI Official Symbol: ACE
• NCBI Official Synonym Symbols: DCP; ICH; ACE1; DCP1; CD143; MVCD3
• NCBI Protein Information: angiotensin-converting enzyme
• UniProt Protein Name: Angiotensin-converting enzyme
• Protein Family: Acetamidase
• UniProt Gene Name: ACE
• UniProt Synonym Gene Names: DCP; DCP1; ACE
• UniProt Entry Name: ACE_HUMAN

NCBI Description

This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010]

Uniprot Description

ACE: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. Genetic variations in ACE may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in ACE are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Genetic variations in ACE are associated with susceptibility to microvascular complications of diabetes type 3 (MVCD3). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in ACE are a cause of susceptibility to intracerebral hemorrhage (ICH). A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Belongs to the peptidase M2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.15.1; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; lysosome; integral to membrane; plasma membrane; extracellular region; endosome; external side of plasma membrane

Molecular Function: tripeptidyl-peptidase activity; peptidyl-dipeptidase activity; zinc ion binding; carboxypeptidase activity; metallopeptidase activity; mitogen-activated protein kinase kinase binding; drug binding; actin binding; protein binding; endopeptidase activity; bradykinin receptor binding; exopeptidase activity; mitogen-activated protein kinase binding; chloride ion binding

Biological Process: mononuclear cell proliferation; regulation of vasodilation; angiotensin mediated regulation of renal output; neutrophil mediated immunity; regulation of angiotensin metabolic process; angiotensin maturation; proteolysis; arachidonic acid secretion; regulation of systemic arterial blood pressure by renin-angiotensin; antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; regulation of smooth muscle cell migration; heart contraction; beta-amyloid metabolic process; regulation of vasoconstriction; regulation of blood pressure; peptide catabolic process; angiotensin catabolic process in blood; spermatogenesis; blood vessel remodeling; hormone catabolic process; kidney development

Disease: Renal Tubular Dysgenesis; Microvascular Complications Of Diabetes, Susceptibility To, 3; Alzheimer Disease; Hemorrhage, Intracerebral, Susceptibility To