Rabbit anti-Human ACACA Polyclonal Antibody | anti-ACACA antibody

ACACA, CT (ACACA, ACAC, ACC1, ACCA, Acetyl-CoA carboxylase 1, ACC-alpha, APC carboxylase) (APC)

Cat. Num. AAA6270750

• Gene Names: ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ACACA; Polyclonal Antibody; CT (ACACA; ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC-alpha; APC carboxylase) (APC); Biotin carboxylase; anti-ACACA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ACACA antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ACACA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2009-2039 amino acids from the C-terminal region of human ACACA.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ACACA Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ACACA antibody detected the ACACA protein (arrow).)

Related Product Information for anti-ACACA antibody

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

Product Categories/Family for anti-ACACA antibody

Antibodies; Enzymes

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Kim, C.W., et al. Proc. Natl. Acad. Sci. U.S.A. 107(21):9626-9631(2010). Zhao, L.F., et al. Endocr. J. 57(4):317-324(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 38679967
• NCBI GeneID: 31
• NCBI Accession #: NP_942133.1; NP_942131.1
• NCBI GenBank Nucleotide #: NM_198836.2
• UniProt Accession #: Q13085; Q6KEV6; Q6XDA8; Q7Z2G8; Q7Z561; Q7Z563; Q7Z564; Q86WB2; Q86WB3; B2RP68
• Molecular Weight: 259,686 Da
• NCBI Official Full Name: acetyl-CoA carboxylase 1 isoform 2
• NCBI Official Synonym Full Names: acetyl-CoA carboxylase alpha
• NCBI Official Symbol: ACACA
• NCBI Official Synonym Symbols: ACC; ACAC; ACC1; ACCA; ACACAD
• NCBI Protein Information: acetyl-CoA carboxylase 1
• UniProt Protein Name: Acetyl-CoA carboxylase 1
• UniProt Gene Name: ACACA
• UniProt Synonym Gene Names: ACAC; ACC1; ACCA; ACC1
• UniProt Entry Name: ACACA_HUMAN

Uniprot Description

ACC1: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC-alpha is the predominant isoform in liver, adipocyte and mammary gland. ACC-beta is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity.

Protein type: Ligase; EC 6.3.4.14; EC 6.4.1.2; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; Lipid Metabolism - fatty acid biosynthesis

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: mitochondrion; cytoplasm; nucleolus; cytosol; actin cytoskeleton

Molecular Function: protein binding; acetyl-CoA carboxylase activity; metal ion binding; biotin carboxylase activity; ATP binding

Biological Process: vitamin metabolic process; acetyl-CoA metabolic process; multicellular organismal protein metabolic process; tissue homeostasis; positive regulation of cellular metabolic process; energy reserve metabolic process; lipid homeostasis; triacylglycerol biosynthetic process; carnitine shuttle; cellular lipid metabolic process; water-soluble vitamin metabolic process; fatty acid biosynthetic process; biotin metabolic process; protein homotetramerization

Disease: Acetyl-coa Carboxylase Deficiency