Goat ABHD4 Polyclonal Antibody | anti-ABHD4 antibody
Goat anti-ABHD4 (aa 304 to 316) Antibody
Gene Names
ABHD4; ABH4
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ABHD4; Polyclonal Antibody; Goat anti-ABHD4 (aa 304 to 316) Antibody; Goat Anti-ABHD4 (aa 177 to 187) Antibody; abhydrolase domain containing 4; FLJ12816; ABHD4 antibody; abhydrolase domain containing 4 antibody; FLJ12816 antibody; ABHD4 (aa 304 to 316); anti-ABHD4 antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QRPDSYVRDMEIK
Sequence Length
342
Applicable Applications for anti-ABHD4 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Preliminary experiments gave an approx 48kDa band in Human Brain (Cerebellum, Cerebellar Cortex, Frontal Cortex) lysates and in Mouse Brain lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.8kDa according to NP_071343.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide.
Western Blot: Preliminary experiments gave an approx 48kDa band in Human Brain (Cerebellum, Cerebellar Cortex, Frontal Cortex) lysates and in Mouse Brain lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.8kDa according to NP_071343.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-QRPDSYVRDMEIK, from the internal region of the protein sequence according to NP_071343.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,239 Da
NCBI Official Full Name
protein ABHD4
NCBI Official Synonym Full Names
abhydrolase domain containing 4
NCBI Official Symbol
ABHD4
NCBI Official Synonym Symbols
ABH4
NCBI Protein Information
protein ABHD4
UniProt Protein Name
Protein ABHD4
Protein Family
UniProt Gene Name
ABHD4
UniProt Synonym Gene Names
Abhydrolase domain-containing protein 4
UniProt Entry Name
ABHD4_HUMAN
Uniprot Description
ABHD4: Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine. Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
Protein type: EC 3.1.1.-; Hydrolase
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: endoplasmic reticulum membrane; lipid particle
Molecular Function: hydrolase activity
Biological Process: lipid catabolic process; lipid metabolic process; phospholipid metabolic process; sequestering of lipid
Research Articles on ABHD4
Similar Products
Product Notes
The ABHD4 abhd4 (Catalog #AAA420869) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ABHD4 (aa 304 to 316) Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's ABHD4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Preliminary experiments gave an approx 48kDa band in Human Brain (Cerebellum, Cerebellar Cortex, Frontal Cortex) lysates and in Mouse Brain lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.8kDa according to NP_071343.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the ABHD4 abhd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QRPDSYVRDM EIK. It is sometimes possible for the material contained within the vial of "ABHD4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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