Rabbit anti-Human ABCB4 Polyclonal Antibody | anti-ABCB4 antibody

Anti-ABCB4 antibody

Cat. Num. AAA176074

• Gene Names: ABCB4; GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: ABCB4; Polyclonal Antibody; Anti-ABCB4 antibody; Multidrug resistance protein 3; ATP-binding cassette; sub-family B(MDR/TAP); member 4; ABC 21 antibody; ABC B4 antibody; ABC21 antibody; ABCB 4 antibody; Abcb4 antibody; ABCB4 protein antibody; ATP binding cassette sub family B MDR/TAP member 4 antibody; ATP binding cassette sub family B member 4 antibody; ATP-binding cassette sub-family B member 4 antibody; MDR 3 antibody; MDR2 antibody; MDR2/3 antibody; MDR3 antibody; MDR3 P glycoprotein antibody; MDR3 P gp antibody; MDR3_HUMAN antibody; Multidrug resistance protein 3 antibody; Multiple drug resistance 3 antibody; P glycoprotein 3 antibody; P-glycoprotein 3 antibody; PFIC 3 antibody; PFIC3 antibody; PGY 3 antibody; PGY3 antibody; anti-ABCB4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 1286

• Applicable Applications for anti-ABCB4 antibody: Western Blot (WB)
• Application Notes: Western Blot:; Concentration:0.1-0.5ug/ml; Tested Species: Hu; ; Tested Species: In-house tested species with positive results.; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; Other applications have not been tested.; Optimal dilutions should be determiend by end users.
• Gene Name: ATP-binding cassette, sub-family B (MDR/TAP), member 4
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ABCB4 (1-20aa MDLEAAKNGTAWRPTSAEGD).
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-ABCB4 antibody, MBS176074, Western blottingWB: MCF-7 Cell Lysate )

Related Product Information for anti-ABCB4 antibody

Description: Rabbit IgG polyclonal antibody for Multidrug resistance protein 3(ABCB4) detection. Tested with WB in Human.
Background: Adenosine triphosphate-binding cassette subfamily B, member 4(ABCB4), also called MDR3, is a protein that in humans is encoded by the ABCB4 gene. MDR1 and MDR3 constitute the human P-glycoprotein gene family. The MDR3 gene contains 28 exons and 27 of these contain coding sequences for the two homologous halves of the protein that correlate with functional domains. MDR3 gene mutations represent a genetic factor involved in this peculiar form of cholesterol gallstone disease in adults.

NCBI and Uniprot Product Information

• NCBI GI #: 126302568
• NCBI GeneID: 5244
• UniProt Accession #: P21439; Q14813; A0A2V7; A4D1D3; A4D1D4; A4D1D5; D6W5P3; D6W5P4
• NCBI Official Full Name: Multidrug resistance protein 3
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 4
• NCBI Official Symbol: ABCB4
• NCBI Official Synonym Symbols: GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
• NCBI Protein Information: multidrug resistance protein 3; P-glycoprotein 3; multiple drug resistance 3; ATP-binding cassette sub-family B member 4; P glycoprotein 3/multiple drug resistance 3; P-glycoprotein-3/multiple drug resistance-3
• UniProt Protein Name: Multidrug resistance protein 3
• Protein Family: ABC transporter B family
• UniProt Gene Name: ABCB4
• UniProt Synonym Gene Names: MDR3; PGY3
• UniProt Entry Name: MDR3_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCB4: a transporter that mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human ABCB4 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. Interacts with HAX1. Defects are the cause of gallstones, and progressive familial intrahepatic cholestasis 3, an autosomal recessive liver disorder that progresses to cirrhosis and liver failure before adulthood. Two alternatively spliced human isoforms have been described.

Protein type: Membrane protein, multi-pass; Transporter, ABC family; Hydrolase; EC 3.6.3.44; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: intercellular canaliculus; membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of substances; xenobiotic-transporting ATPase activity; ATP binding

Biological Process: response to drug; transport; multidrug transport; xenobiotic transport; lipid metabolic process; cellular lipid metabolic process; transmembrane transport

Disease: Gallbladder Disease 1; Cholestasis, Progressive Familial Intrahepatic, 3; Cholestasis, Intrahepatic, Of Pregnancy 3