Rabbit anti-Human A2M Polyclonal Antibody | anti-A2M antibody

A2M, NT (A2M, CPAMD5, Alpha-2-macroglobulin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5) (Azide free) (HRP)

Cat. Num. AAA6270280

• Gene Names: A2M; A2MD; CPAMD5; FWP007; S863-7
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: A2M; Polyclonal Antibody; NT (A2M; CPAMD5; Alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5) (Azide free) (HRP); C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; anti-A2M antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-A2M antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: A2M antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-120 amino acids from the N-terminal region of human A2M.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(A2M Antibody (N-term) western blot analysis in mouse plasma lysates (35ug/lane).This demonstrates the A2M antibody detected the A2M protein (arrow).)

Related Product Information for anti-A2M antibody

Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq].

Product Categories/Family for anti-A2M antibody

Antibodies; Protease Inhibitors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Bruno, E., et al. Neurosci. Lett. 482(2):112-116(2010). Nalpas, B., et al. Gut 59(8):1120-1126(2010). Song, H., et al. Neurosci. Lett. 479(2):143-145(2010). Seriramalu, R., et al. Electrophoresis 31(14):2388-2395(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 66932947
• NCBI GeneID: 2
• NCBI Accession #: NP_000005.2
• NCBI GenBank Nucleotide #: NM_000014.4
• UniProt Accession #: P01023; Q13677; Q59F47; Q5QTS0; Q68DN2; Q6PIY3; Q6PN97
• Molecular Weight: 163,291 Da
• NCBI Official Full Name: alpha-2-macroglobulin
• NCBI Official Synonym Full Names: alpha-2-macroglobulin
• NCBI Official Symbol: A2M
• NCBI Official Synonym Symbols: A2MD; CPAMD5; FWP007; S863-7
• NCBI Protein Information: alpha-2-macroglobulin; alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
• UniProt Protein Name: Alpha-2-macroglobulin
• Protein Family: Alpha-2-macroglobulin
• UniProt Gene Name: A2M
• UniProt Synonym Gene Names: CPAMD5; Alpha-2-M
• UniProt Entry Name: A2MG_HUMAN

NCBI Description

Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]

Uniprot Description

A2M: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: extracellular region; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-1 binding; interleukin-8 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding

Biological Process: negative regulation of complement activation, lectin pathway; platelet activation; extracellular matrix disassembly; regulation of small GTPase mediated signal transduction; extracellular matrix organization and biogenesis; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway

Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency