Rabbit anti-Human A-RAF Polyclonal Antibody | anti-A-RAF antibody

A-RAF, ID (Serine/Threonine-protein Kinase A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene A-Raf, Proto-oncogene Pks, ARAF, ARAF1, PKS, PKS2) (MaxLight 650)

Cat. Num. AAA6496570

• Gene Names: ARAF; PKS2; A-RAF; ARAF1; RAFA1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: A-RAF; Polyclonal Antibody; ID (Serine/Threonine-protein Kinase A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene A-Raf; Proto-oncogene Pks; ARAF; ARAF1; PKS; PKS2) (MaxLight 650); anti-A-RAF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human A-RAF.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight650.

• Applicable Applications for anti-A-RAF antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 256-286 in the central region of human A-RAF, UniProt Accession #P10398.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MCF7 cell line lysates (35ug/lane) usingMBS625306. ARAF1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS625306, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-A-RAF antibody

Antibodies; Protein Kinases

References

1. Wu, X., et al., J. Biol. Chem. 271(6):3265-3271 (1996). 2. Lee, J.E., et al., Genomics 20(1):43-55 (1994). 3. Popescu, N.C., et al., Oncogene 4(4):517-519 (1989). 4. Beck, T.W., et al., Nucleic Acids Res. 15(2):595-609 (1987). 5. Yin, X.L., et al., Mol. Cell. Biochem. 231(1-2), 69-74 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 371875793
• NCBI GeneID: 369
• NCBI Accession #: NP_001243125.1
• NCBI GenBank Nucleotide #: NM_001256196.1
• UniProt Accession #: P10398; P07557; Q5H9B2; Q5H9B3
• Molecular Weight: 67,585 Da
• NCBI Official Full Name: serine/threonine-protein kinase A-Raf isoform 2
• NCBI Official Synonym Full Names: v-raf murine sarcoma 3611 viral oncogene homolog
• NCBI Official Symbol: ARAF
• NCBI Official Synonym Symbols: PKS2; A-RAF; ARAF1; RAFA1
• NCBI Protein Information: serine/threonine-protein kinase A-Raf; Oncogene ARAF1; proto-oncogene Pks; proto-oncogene A-Raf-1; Ras-binding protein DA-Raf; v-raf murine sarcoma 3611 viral oncogene homolog 1; A-Raf proto-oncogene serine/threonine-protein kinase
• UniProt Protein Name: Serine/threonine-protein kinase A-Raf
• UniProt Gene Name: ARAF
• UniProt Synonym Gene Names: ARAF1; PKS; PKS2
• UniProt Entry Name: ARAF_HUMAN

NCBI Description

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]

Uniprot Description

ARAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib.

Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Oncoprotein; Kinase, protein; EC 2.7.11.1; TKL group; RAF family

Chromosomal Location of Human Ortholog: Xp11.4-p11.2

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; receptor signaling protein activity; ATP binding; protein kinase activity

Biological Process: regulation of proteasomal ubiquitin-dependent protein catabolic process; protein modification process; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; negative regulation of apoptosis; regulation of TOR signaling pathway