Western Blot (WB)
(Staining (0. 01ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat 58KGolgi protein Polyclonal Antibody | anti-FTCD antibody
Goat anti-58KGolgi protein(Internal)/FTCD Antibody
Immunohistochemistry: In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration, 3-5ug/ml.
Western Blot: Approx 60kDa band observed in Human Liver lysates (calculated MW of 58.9kDa according to NP_006648.1and NP_996848.1). Recommended concentration: 0.01-0.03ug/ml.
Western Blot (WB)
(Staining (0. 01ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0. 01ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Dec 2009]
Uniprot Description
FTCD: Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA); also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - histidine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.2.5; EC 4.3.1.4; Lyase; Methyltransferase
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; ER-Golgi intermediate compartment; Golgi apparatus; Golgi membrane; smooth endoplasmic reticulum membrane
Molecular Function: formimidoyltetrahydrofolate cyclodeaminase activity; formimidoyltransferase activity; microtubule binding; protein binding
Biological Process: folic acid and derivative metabolic process; histidine catabolic process
Disease: Formiminotransferase Deficiency
Research Articles on FTCD
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Product Notes
The FTCD ftcd (Catalog #AAA420986) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-58KGolgi protein(Internal)/FTCD Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's 58KGolgi protein can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration, 3-5ug/ml. Western Blot: Approx 60kDa band observed in Human Liver lysates (calculated MW of 58.9kDa according to NP_006648.1and NP_996848.1). Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the FTCD ftcd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CLREQGRGKD QPGRL. It is sometimes possible for the material contained within the vial of "58KGolgi protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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