Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse 5'-Nucleotidase, Cytosolic III (NT5C3) Polyclonal Antibody | anti-NT5C3 antibody
Polyclonal Antibody to 5'-Nucleotidase, Cytosolic III (NT5C3)
Gene Names
Nt5c3; p36; Nt5y; PN-1; PN-I; PSN1; UMPH; Umph1; lupin; Nt5c3a; Umph-1; cN-III; AI842776; 1600024P05Rik; 2610206B05Rik; 3110004A18Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
5'-Nucleotidase; Cytosolic III (NT5C3); Polyclonal Antibody; Polyclonal Antibody to 5'-Nucleotidase; anti-NT5C3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against NT5C3. It has been selected for its ability to recognize NT5C3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGSEF-MTNQESAVHL KMMPEFQKSS VRIKNPTRVE EIICGLIKGG AAKLQIITDF DMTLSRFSYN GKRCPTCHNI IDNCKLVTDE CRRKLLQLKE QYYAIEVDPV LTVEEKFPYM VEWYTKSHGL LIEQGIPKAK LKEIVADSDV MLKEGYENFF GKLQQHGIPV FIFSAGIGDV LEEVIRQAGV YHSNVKVVSN FMDFDENGVL KGFKGELIHV FNKHDGALKN TDYFSQLKDN SNIILLGDSQ GDLRMADGVA NVEHILKIGY LNDRVDELLE KYMDSYDIVL VKEESLEVVN SILQKTL
MGHHHHHHSGSEF-MTNQESAVHL KMMPEFQKSS VRIKNPTRVE EIICGLIKGG AAKLQIITDF DMTLSRFSYN GKRCPTCHNI IDNCKLVTDE CRRKLLQLKE QYYAIEVDPV LTVEEKFPYM VEWYTKSHGL LIEQGIPKAK LKEIVADSDV MLKEGYENFF GKLQQHGIPV FIFSAGIGDV LEEVIRQAGV YHSNVKVVSN FMDFDENGVL KGFKGELIHV FNKHDGALKN TDYFSQLKDN SNIILLGDSQ GDLRMADGVA NVEHILKIGY LNDRVDELLE KYMDSYDIVL VKEESLEVVN SILQKTL
Sequence Length
336
Applicable Applications for anti-NT5C3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant NT5C3 (Met1~Leu297) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2073663
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded stomach tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded stomach tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,790 Da
NCBI Official Full Name
cytosolic 5'-nucleotidase 3A isoform 2
NCBI Official Synonym Full Names
5'-nucleotidase, cytosolic III
NCBI Official Symbol
Nt5c3
NCBI Official Synonym Symbols
p36; Nt5y; PN-1; PN-I; PSN1; UMPH; Umph1; lupin; Nt5c3a; Umph-1; cN-III; AI842776; 1600024P05Rik; 2610206B05Rik; 3110004A18Rik
NCBI Protein Information
cytosolic 5'-nucleotidase 3A
UniProt Protein Name
Cytosolic 5'-nucleotidase 3A
Protein Family
UniProt Gene Name
Nt5c3a
UniProt Synonym Gene Names
Nt5c3; 7-methylguanosine nucleotidase; cN-III; P5'N-1; P5N-1; PN-I
Uniprot Description
NT5C3A: Can act both as nucleotidase and as phosphotransferase. Defects in NT5C3 are the cause of P5N deficiency (P5ND); also called hemolytic anemia due to P5N deficiency or hemolytic anemia due to UMPH1 deficiency. P5ND is an autosomal recessive condition causing hemolytic anemia characterized by marked basophilic stipplig and the accumulation of high concentrations of pyrimidine nucleotides within the erythrocyte. It is implicated in the anemia of lead poisoning and is possibly associated with learning difficulties. Belongs to the pyrimidine 5'-nucleotidase family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.1.3.5; Endoplasmic reticulum; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Phosphatase (non-protein); Transferase
Chromosomal Location of Human Ortholog: 6|6 B3
Cellular Component: cytoplasm; endoplasmic reticulum; mitochondrion
Molecular Function: 5'-nucleotidase activity; hydrolase activity; magnesium ion binding; metal ion binding; nucleotide binding; transferase activity
Biological Process: adenosine metabolic process; defense response to virus; nucleotide metabolic process
Research Articles on NT5C3
Similar Products
Product Notes
The NT5C3 nt5c3a (Catalog #AAA2003007) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to 5'-Nucleotidase, Cytosolic III (NT5C3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 5'-Nucleotidase, Cytosolic III (NT5C3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NT5C3 nt5c3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-MTNQES AVHL KMMPEFQKSS VRIKNPTRVE EIICGLIKGG AAKLQIITDF DMTLSRFSYN GKRCPTCHNI IDNCKLVTDE CRRKLLQLKE QYYAIEVDPV LTVEEKFPYM VEWYTKSHGL LIEQGIPKAK LKEIVADSDV MLKEGYENFF GKLQQHGIPV FIFSAGIGDV LEEVIRQAGV YHSNVKVVSN FMDFDENGVL KGFKGELIHV FNKHDGALKN TDYFSQLKDN SNIILLGDSQ GDLRMADGVA NVEHILKIGY LNDRVDELLE KYMDSYDIVL VKEESLEVVN SILQKTL. It is sometimes possible for the material contained within the vial of "5'-Nucleotidase, Cytosolic III (NT5C3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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