Immunofluorescence
(Immunofluorescent staining of 5-Methyl Cytosine antibodyPositive staining of rat neuron cells blocked with 2% BSA/1% Tween-20 in PBS & incubated at 4C overnight with an antibody dilution of (5 ug/ml))
Sheep 5-Methyl Cytosine Polyclonal Antibody
5-Methyl Cytosine antibody
Reactivity
To be determined by end-user
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
5-Methyl Cytosine; Polyclonal Antibody; 5-Methyl Cytosine antibody; Polyclonal 5-Methyl Cytosine; Anti-5-Methyl Cytosine; 5MeC; -Methyl Cytosine 5; -Methyl Cytosine-5; 5MC; anti-5-Methyl Cytosine antibody
Host
Sheep
Reactivity
To be determined by end-user
Clonality
Polyclonal
Form/Format
Purified IgG supplied in 150mM PBS, pH 7.4 with no preservatives added.
Concentration
Typically 1-5 mg/ml (varies by lot)
Applicable Applications for anti-5-Methyl Cytosine antibody
Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IHC: 10 ug/ml
IP: 25-100 ug/ml
WB: 1:1,000
IP: 25-100 ug/ml
WB: 1:1,000
Biological Significance
5-Methylcytosine is a methylated form of the DNA base cytosine. When cytosine is methylated, the DNA maintains the same sequence, but genes can be turned on and off (the study of this is called epigenetics).
Immunogen
5-Methyl cytosine antibody was raised in sheep using 5-methyl cytosine as the immunogen.
Preparation and Storage
Aliquot and store at -20 degree C. Avoid repeated Freeze/Thaw cycles.
Immunofluorescence
(Immunofluorescent staining of 5-Methyl Cytosine antibodyPositive staining of rat neuron cells blocked with 2% BSA/1% Tween-20 in PBS & incubated at 4C overnight with an antibody dilution of (5 ug/ml))
Immunofluorescence
(Immunofluorescent staining of 5-Methyl Cytosine antibodyPositive staining of rat neuron cells blocked with 2% BSA/1% Tween-20 in PBS & incubated at 4C overnight with an antibody dilution of (5 ug/ml))
Related Product Information for anti-5-Methyl Cytosine antibody
Sheep polyclonal 5-Methyl Cytosine antibody
Product Categories/Family for anti-5-Methyl Cytosine antibody
Similar Products
Product Notes
The 5-Methyl Cytosine (Catalog #AAA534787) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The 5-Methyl Cytosine antibody reacts with To be determined by end-user and may cross-react with other species as described in the data sheet. AAA Biotech's 5-Methyl Cytosine can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). IHC: 10 ug/ml IP: 25-100 ug/ml WB: 1:1,000. Researchers should empirically determine the suitability of the 5-Methyl Cytosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5-Methyl Cytosine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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