Western Blot (WB)
(Western blot analysis of 14-3-3 zeta (pT232) expression in A431 UV-treated (A), K562 UV-treated (B), NIH3T3 UV-treated (C) whole cell lysates.)
Rabbit 14-3-3 zeta Polyclonal Antibody | anti-YWHAZ antibody
Anti-14-3-3 zeta (pT232) Phospho Antibody
Western Blot (WB)
(Western blot analysis of 14-3-3 zeta (pT232) expression in A431 UV-treated (A), K562 UV-treated (B), NIH3T3 UV-treated (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of 14-3-3 zeta (pT232) expression in A431 UV-treated (A), K562 UV-treated (B), NIH3T3 UV-treated (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of 14-3-3 zeta (pT232) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of 14-3-3 zeta (pT232) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]
Uniprot Description
14-3-3 zeta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Phosphorylation apparently disrupts homodimerization.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 8q23.1
Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; extracellular space; focal adhesion; melanosome; mitochondrion; nucleoplasm; nucleus
Molecular Function: identical protein binding; protein binding; protein domain specific binding; protein kinase binding; transcription factor binding; ubiquitin protein ligase binding
Biological Process: apoptosis; blood coagulation; establishment of Golgi localization; gene expression; negative regulation of apoptosis; platelet activation; programmed cell death; protein targeting; regulation of mRNA stability; signal transduction; small GTPase mediated signal transduction; transcription initiation from RNA polymerase II promoter
Research Articles on YWHAZ
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Product Notes
The YWHAZ ywhaz (Catalog #AAA8242990) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-14-3-3 zeta (pT232) Phospho Antibody reacts with Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 zeta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the YWHAZ ywhaz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 zeta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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