POLR2J3 recombinant protein

POLR2J3, 1-115aa, Human, His tag, E Coli

Cat. Num. AAA204625

• Gene Names: POLR2J3; POLR2J2; RPB11b1; RPB11b2
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: POLR2J3; 1-115aa; Human; His tag; E Coli; DNA-directed RNA polymerase II subunit RPB11-b2; POLR2J2; RPB11b1; RPB11b2; POLR2J3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK ITINKDTKVP NACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP
• Sequence Length: 115

• Applicable Applications for POLR2J3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for POLR2J3 recombinant protein

DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2J3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for POLR2J3 recombinant protein

Epigenomics

References

Palangat M., et al. (2004) J Mol Biol. 341:429-442; uivari A., et al. (2006) Nat Struct Mol Biol. 13: 49-54.

NCBI and Uniprot Product Information

• NCBI GI #: 332634984
• NCBI GeneID: 548644
• NCBI Accession #: NP_001091084
• NCBI GenBank Nucleotide #: NM_001097615.2
• Molecular Weight: 15.5 kDa (138aa) confirmed by MALDI-TOF
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB11-b2
• NCBI Official Synonym Full Names: RNA polymerase II subunit J3
• NCBI Official Symbol: POLR2J3
• NCBI Official Synonym Symbols: POLR2J2; RPB11b1; RPB11b2
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB11-b2
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB11-b2
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: POLR2J3
• UniProt Synonym Gene Names: RNA polymerase II subunit B11-b2; RPB11b2

NCBI Description

This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq, Jul 2008]

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft ().