Polymerase II Polypeptide I Recombinant Protein | POLR2I recombinant protein

Recombinant Human Polymerase II Polypeptide I

Cat. Num. AAA146311

• Gene Names: POLR2I; RPB9; hRPB14.5
• Purity: Greater than 85% as determined by SDS-PAGE.
• Synonyms: Polymerase II Polypeptide I; Recombinant Human Polymerase II Polypeptide I; POLR2I Human; Polymerase II Polypeptide I Human Recombinant; hRPB14.5; RPB9; DNA-directed RNA polymerase II subunit RPB9; DNA-directed RNA polymerase II subunit I; RNA polymerase II 14.5 kDa subunit; RPB14.5; POLR2I recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85% as determined by SDS-PAGE.
• Form/Format: POLR2I protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 20% glycerol and 1mM DTT.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEPDGTY EPGFVGIRFC QECNNMLYPK EDKENRILLY ACRNCDYQQE ADNSCIYVNK ITHEVDELTQ IIADVSQDPT LPRTEDHPCQ KCGHKEAVFF QSHSARAEDA MRLYYVCTAP HCGHRWTE
• Sequence Length: 125

Related Product Information for POLR2I recombinant protein

Description: POLR2I Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 148 amino acids (1-125 a.a.) and having a molecular mass of 17.0kDa.POLR2I is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Polymerase II Polypeptide I (POLR2I) is a member of the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. POLR2I is a subunit of RNA polymerase II, which is a polymerase responsible for synthesizing messenger RNA in eukaryotes. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the 4 ribonucleoside triphosphates as substrates. In addition POLR2I synthesizes mRNA precursors and numerous functional non-coding RNAs.

Product Categories/Family for POLR2I recombinant protein

Enzymes; Polymerase

NCBI and Uniprot Product Information

• NCBI GI #: 5453930
• NCBI GeneID: 5438
• NCBI Accession #: NP_006224.1
• NCBI GenBank Nucleotide #: NM_006233.4
• UniProt Accession #: P36954; Q6NW05; B2R5J2
• Molecular Weight: 14,523 Da
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB9
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
• NCBI Official Symbol: POLR2I
• NCBI Official Synonym Symbols: RPB9; hRPB14.5
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB9; DNA-directed RNA polymerase II 14.5 kDa polypeptide; DNA-directed RNA polymerase II subunit I; RNA polymerase II 14.5 kDa subunit; RNA polymerase II subunit B9; RPB14.5
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB9
• UniProt Gene Name: POLR2I
• UniProt Synonym Gene Names: RNA polymerase II subunit B9; RPB14.5
• UniProt Entry Name: RPB9_HUMAN

NCBI Description

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq, Jul 2008]

Uniprot Description

POLR2I: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template. Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.

Protein type: Transferase; EC 2.7.7.6; Nucleotide Metabolism - pyrimidine; Transcription initiation complex; Nucleotide Metabolism - purine; RNA processing

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function: DNA binding; zinc ion binding; DNA-directed RNA polymerase activity

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; somatic stem cell maintenance; positive regulation of viral transcription; nucleotide-excision repair; RNA splicing; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair