Rat DNA polymerase epsilon subunit 3 ELISA Kit | POLE3 elisa kit
Rat DNA polymerase epsilon subunit 3 (POLE3) ELISA Kit
Reactivity
Rat
Synonyms
DNA polymerase epsilon subunit 3; Rat DNA polymerase epsilon subunit 3 (POLE3) ELISA Kit; CHARAC17; CHRAC17; YBL1; p17; DNA polymerase epsilon p17 subunit|DNA-directed DNA polymerase epsilon 3|arsenic transactivated protein|chromatin accessibility complex 17|histone fold protein CHRAC17; POLE3 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat POLE3. No significant cross-reactivity or interference between Rat POLE3 and analogues was observed.
Sequence Length
147
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for POLE3 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate POLE3 in samples. An antibody specific for POLE3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLE3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLE3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLE3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,674 Da
NCBI Official Full Name
DNA polymerase epsilon subunit 3
NCBI Official Synonym Full Names
polymerase (DNA directed), epsilon 3, accessory subunit
NCBI Official Symbol
Pole3
NCBI Protein Information
DNA polymerase epsilon subunit 3
UniProt Protein Name
DNA polymerase epsilon subunit 3
Protein Family
UniProt Gene Name
Pole3
Uniprot Description
POLE3: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
Protein type: DNA replication; EC 2.7.7.7; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transferase
Chromosomal Location of Human Ortholog: 5q24
Cellular Component: epsilon DNA polymerase complex
Molecular Function: DNA binding; DNA-directed DNA polymerase activity; protein heterodimerization activity
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Product Notes
The Rat POLE3 pole3 (Catalog #AAA283771) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283771 ELISA Kit recognizes Rat POLE3. It is sometimes possible for the material contained within the vial of "DNA polymerase epsilon subunit 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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