SDS-PAGE
Thermus aquacicus DNA polymerase I, thermostable Recombinant Protein | pol1 recombinant protein
Recombinant thermus aquacicus DNA polymerase I, thermostable
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Thermus aquacicus DNA polymerase I; thermostable; Recombinant thermus aquacicus DNA polymerase I; Taq polymerase 1; pol1 recombinant protein
Host
E Coli
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Liquid containing glycerol
Sequence Positions
289-832aa; Partial
Sequence
ESPKALEEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKALRDLKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVARRYGGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLAHMEATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLERVLFDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLKSTYIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRIRRAFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDIHTETASWMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAFIERYFQSFPKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVREAAERMAFNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAPKERAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE
Sequence Length
832
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
References
Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus.Lawyer F.C., Stoffel S., Saiki R.K., Myambo K., Drummond R., Gelfand D.H.J. Biol. Chem. 264:6427-6437(1989)
Overproduction of Thermus aquaticus DNA polymerase and its structural analysis by ion-spray mass spectrometry.Ishino Y., Ueno T., Miyagi M., Uemori T., Imamura M., Tsunasawa S., Kato I.J. Biochem. 116:1019-1024(1994)
Crystal structure of Thermus aquaticus DNA polymerase.Kim Y., Eom S.H., Wang J., Lee D.-S., Suh S.W., Steitz T.A.Nature 376:612-616(1995)
Crystal structure of the large fragment of Thermus aquaticus DNA polymerase I at 2.5-A resolution
structural basis for thermostability.Korolev S., Nayal M., Barnes W.M., di Cera E., Waksman G.Proc. Natl. Acad. Sci. U.S.A. 92:9264-9268(1995)
Structure of Taq polymerase with DNA at the polymerase active site.Eom S.H., Wang J., Steitz T.A.Nature 382:278-281(1996)
Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I
structural basis for nucleotide incorporation.Li Y., Korolev S., Waksman G.EMBO J. 17:7514-7525(1998)
Crystal structures of the Klenow fragment of Thermus aquaticus DNA polymerase I complexed with deoxyribonucleoside triphosphates.Li Y., Kong Y., Korolev S., Waksman G.Protein Sci. 7:1116-1123(1998)
Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation.Li Y., Mitaxov V., Waksman G.Proc. Natl. Acad. Sci. U.S.A. 96:9491-9496(1999)
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
65.3 kDa
NCBI Official Full Name
DNA polymerase I, thermostable
UniProt Protein Name
DNA polymerase I, thermostable
Protein Family
UniProt Gene Name
polA
UniProt Synonym Gene Names
pol1
UniProt Entry Name
DPO1_THEAQ
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Product Notes
The pol1 pola (Catalog #AAA1265573) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 289-832aa; Partial. The amino acid sequence is listed below: ESPKALEEAP WPPPEGAFVG FVLSRKEPMW ADLLALAAAR GGRVHRAPEP YKALRDLKEA RGLLAKDLSV LALREGLGLP PGDDPMLLAY LLDPSNTTPE GVARRYGGEW TEEAGERAAL SERLFANLWG RLEGEERLLW LYREVERPLS AVLAHMEATG VRLDVAYLRA LSLEVAEEIA RLEAEVFRLA GHPFNLNSRD QLERVLFDEL GLPAIGKTEK TGKRSTSAAV LEALREAHPI VEKILQYREL TKLKSTYIDP LPDLIHPRTG RLHTRFNQTA TATGRLSSSD PNLQNIPVRT PLGQRIRRAF IAEEGWLLVA LDYSQIELRV LAHLSGDENL IRVFQEGRDI HTETASWMFG VPREAVDPLM RRAAKTINFG VLYGMSAHRL SQELAIPYEE AQAFIERYFQ SFPKVRAWIE KTLEEGRRRG YVETLFGRRR YVPDLEARVK SVREAAERMA FNMPVQGTAA DLMKLAMVKL FPRLEEMGAR MLLQVHDELV LEAPKERAEA VARLAKEVME GVYPLAVPLE VEVGIGEDWL SAKE. It is sometimes possible for the material contained within the vial of "Thermus aquacicus DNA polymerase I, thermostable, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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