Human Podocalyxin ELISA Kit | PODXL elisa kit

Human Podocalyxin ELISA Kit

Cat. Num. AAA765311

• Gene Names: Podxl; PC; PCLP-1; podocalyxin
• Reactivity: Human
• Synonyms: Podocalyxin; Human Podocalyxin ELISA Kit; PCX (Podocalyxin)/PODXL/PCLP/PCLP1/Podocalyxin-like protein 1/PC/PCLP-1/GCTM-2 antigen/Gp200; PODXL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PODXL. No significant cross-reactivity or interference between PODXL and analogues was observed.
• Sequence Length: 485

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for PODXL elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 20301990
• NCBI GeneID: 192181
• NCBI Accession #: NP_620203.1
• NCBI GenBank Nucleotide #: NM_138848.1
• Molecular Weight: 51,658 Da
• NCBI Official Full Name: podocalyxin
• NCBI Official Synonym Full Names: podocalyxin-like
• NCBI Official Symbol: Podxl
• NCBI Official Synonym Symbols: PC; PCLP-1; podocalyxin
• NCBI Protein Information: podocalyxin
• UniProt Protein Name: Podocalyxin
• Protein Family: Podocalyxin
• UniProt Gene Name: Podxl
• UniProt Synonym Gene Names: Pclp1; PC; PCLP-1
• UniProt Entry Name: PODXL_RAT

NCBI Description

inhibits cell-cell adhesion; may play a role in maintaining filtration in the glomerular epithelium [RGD, Feb 2006]

Uniprot Description

PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: apical plasma membrane; cell projection; cytoplasm; extracellular space; filopodium; integral to membrane; intracellular membrane-bound organelle; lamellipodium; lipid raft; microvillus membrane; plasma membrane; ruffle

Molecular Function: protein binding

Biological Process: cell adhesion; cell migration; glomerular filtration; leukocyte migration; negative regulation of cell adhesion; negative regulation of cell-cell adhesion; positive regulation of cell migration; positive regulation of cell-cell adhesion mediated by integrin; regulation of cell-cell adhesion; regulation of microvillus biogenesis