Standard Curve (Sample)
Goat Caspase 14 ELISA Kit | Caspase-14 elisa kit
Goat Caspase 14 ELISA Kit
Reactivity
Goat
Synonyms
Caspase 14; N/A; Goat Caspase 14 ELISA Kit; Caspase-14 elisa kit
Reactivity
Goat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Caspase-14 elisa kit
Intended Uses: This CASP14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat CASP14. This ELISA kit for research use only!
Principle of the Assay: CASP14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CASP14 antibody and an CASP14-HRP conjugate. The assay sample and buffer are incubated together with CASP14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CASP14 concentration since CASP14 from samples and CASP14-HRP conjugate compete for the anti-CASP14 antibody binding site. Since the number of sites is limited, as more sites are occupied by CASP14 from the sample, fewer sites are left to bind CASP14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASP14 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CASP14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CASP14 antibody and an CASP14-HRP conjugate. The assay sample and buffer are incubated together with CASP14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CASP14 concentration since CASP14 from samples and CASP14-HRP conjugate compete for the anti-CASP14 antibody binding site. Since the number of sites is limited, as more sites are occupied by CASP14 from the sample, fewer sites are left to bind CASP14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASP14 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Caspase-14 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,680 Da
NCBI Official Full Name
caspase-14
NCBI Official Synonym Full Names
caspase 14, apoptosis-related cysteine peptidase
NCBI Official Symbol
CASP14
NCBI Protein Information
caspase-14; CASP-14; apoptosis-related cysteine protease; caspase 14, apoptosis-related cysteine protease
UniProt Protein Name
Caspase-14
UniProt Gene Name
CASP14
UniProt Synonym Gene Names
CASP-14
UniProt Entry Name
CASPE_HUMAN
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Product Notes
The Goat Caspase-14 casp14 (Catalog #AAA93190) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA93190 ELISA Kit recognizes Goat Caspase-14. It is sometimes possible for the material contained within the vial of "Caspase 14, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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