Human Prepronociceptin ELISA Kit | PNOC elisa kit

Human Prepronociceptin ELISA Kit

Cat. Num. AAA763663

• Gene Names: Pnoc; N23K; Npnc1
• Reactivity: Human
• Synonyms: Prepronociceptin; Human Prepronociceptin ELISA Kit; PNOC/OFQ/Prepronociceptin; PNOC elisa kit

• Reactivity: Human
• Sequence Length: 181

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PNOC elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PNOC. During the reaction, PNOC in the sample or standard competes with a fixed amount of biotin-labeled PNOC for sites on a pre-coated Monoclonal antibody specific to PNOC. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PNOC in the samples is then determined by comparing the OD. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6981378
• NCBI GeneID: 25516
• NCBI Accession #: NP_037139.1
• NCBI GenBank Nucleotide #: NM_013007.1
• Molecular Weight: 20,479 Da
• NCBI Official Full Name: prepronociceptin
• NCBI Official Synonym Full Names: prepronociceptin
• NCBI Official Symbol: Pnoc
• NCBI Official Synonym Symbols: N23K; Npnc1
• NCBI Protein Information: prepronociceptin
• UniProt Protein Name: Prepronociceptin
• Protein Family: Prepronociceptin
• UniProt Gene Name: Pnoc
• UniProt Entry Name: PNOC_RAT

NCBI Description

endogenous ligand of the opioid-like orphan receptor; reduces neuronal excitability and induces an increase in inward rectifying K+ conductance in thalamic neurons [RGD, Feb 2006]

Uniprot Description

Nociceptin: Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.