Bifunctional polynucleotide phosphatase/kinase Recombinant Protein | PNKP recombinant protein

Recombinant Human Bifunctional polynucleotide phosphatase/kinase

Cat. Num. AAA9421358

• Gene Names: PNKP; PNK; AOA4; MCSZ; EIEE10
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Bifunctional polynucleotide phosphatase/kinase; Recombinant Human Bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatasePolynucleotide kinase-3'-phosphatase 2 domains:Polynucleotide 3'-phosphatase (EC:3.1.3.32); 2' (3')-polynucleotidasePolynucleotide 5'-hydroxyl-kinase (EC:2.7.1.78); PNKP recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Full Length, 1-521aa

• Tag Info: His-SUMO-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for PNKP recombinant protein

Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either roving 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.

NCBI and Uniprot Product Information

• NCBI GI #: 31543419
• NCBI GeneID: 11284
• NCBI Accession #: NP_009185.2
• NCBI GenBank Nucleotide #: NM_007254.3
• UniProt Accession #: Q96T60; Q9BUL2; Q9P1V2; Q9UKU8; Q9UNF8; Q9UNI0
• Molecular Weight: 73kD
• NCBI Official Full Name: bifunctional polynucleotide phosphatase/kinase
• NCBI Official Synonym Full Names: polynucleotide kinase 3'-phosphatase
• NCBI Official Symbol: PNKP
• NCBI Official Synonym Symbols: PNK; AOA4; MCSZ; EIEE10
• NCBI Protein Information: bifunctional polynucleotide phosphatase/kinase
• UniProt Protein Name: Bifunctional polynucleotide phosphatase/kinase
• Protein Family: Bifunctional polynucleotide phosphatase/kinase
• UniProt Gene Name: PNKP

NCBI Description

This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010]

Uniprot Description

Pnk1: polynucleotide kinase 3'-phosphatase. May be involved in double-strand break repair by non-homologous end joining. Required for normal response to DNA damage by gamma-radiation or camptothecin in S. pombe.

Protein type: DNA-binding; EC 2.7.1.78; EC 3.1.3.32; Kinase, other; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: membrane; nucleolus; nucleoplasm; nucleus

Molecular Function: ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; double-stranded DNA binding; nucleotide kinase activity; polynucleotide 3'-phosphatase activity; protein binding

Biological Process: dephosphorylation; DNA damage response, detection of DNA damage; DNA repair; nucleotide phosphorylation; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; response to oxidative stress; response to radiation

Disease: Ataxia-oculomotor Apraxia 4; Microcephaly, Seizures, And Developmental Delay