SDS-Page
PM20D1 Recombinant Protein | IL2RA recombinant protein
Human CellExp PM20D1, Human Recombinant
Purity
>95%
Synonyms
PM20D1; Human CellExp PM20D1; Human Recombinant; N-fatty-acyl-amino acid synthase; hydrolase PM20D1; Peptidase M20 domain-containing protein 1; IL2RA recombinant protein
Host
HEK293 cells
Purity/Purification
>95%
Form/Format
Lyophilized from 0.22um filtered PBS, pH7.4 with 5% trehalose
Gene Source
Human
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in sterile water to desired concentration. Do not vortex.
Specific activity
PM20D1 Specific Activity: Different amount of PM20D1 was assayed in a colorimetric assay, where PM20D1’s ability to hydrolyze substrate N-Oleo-Glycine was measured. The specific activity is >200 mU/mg. One unit of PM20D1 is defined as the amount of enzyme that generates 1.0 umol of glycine per min under the assay condition at 37°C.
Unit definition
One unit of PM20D1 is defined as the amount of enzyme that generates 1.0 ?mol of glycine per min under the assay condition at 37°C.
Preparation and Storage
Store at -20°C. After reconstitution, aliquot and store at -80°C and use within 3 months. Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Testing Data
Testing Data
Related Product Information for IL2RA recombinant protein
PM20D1 is a recently discovered (2016) enzyme that regulates lipidated amino acid uncouplers of mitochondria. PM20D1 is a secreted and bidirectional enzyme that can generate N-acyl amino acids (NAAs) from amino acids and free fatty acids; it can also hydrolyze NAAs into amino acids and free fatty acids. NAAs can directly bind mitochondria and function as endogenous uncouplers of UCP1-independent respiration. Mouse and human PM20D1 share 71% amino acid identity. Both contain signal peptides and have identical catalytic residues. PM20D1 may be useful in treating obesity and obesity-related disorders. Other studies suggest that PM20D1 is also associated with early childhood wheezing. Ablation of PM20D1 using mouse model reveals physiologic functions of PM20D1 in NAA signaling of metabolism and nociception.
Product Categories/Family for IL2RA recombinant protein
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
Molecular Weight
~60 kDa (with glycosylation and C terminal 8× His tag)
Protein Family
Similar Products
Product Notes
The IL2RA (Catalog #AAA8400053) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PM20D1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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