Human Plexin-A2 ELISA Kit | PLXNA2 elisa kit

Human Plexin-A2 (PLXNA2) ELISA Kit

Cat. Num. AAA280969

• Gene Names: PLXNA2; OCT; PLXN2
• Reactivity: Human
• Synonyms: Plexin-A2; Human Plexin-A2 (PLXNA2) ELISA Kit; PLXNA2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PLXNA2. No significant cross-reactivity or interference between Human PLXNA2 and analogues was observed.
• Sequence Length: 1894

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PLXNA2 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLXNA2 in samples. An antibody specific for PLXNA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLXNA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXNA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXNA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 113722116
• NCBI GeneID: 5362
• NCBI Accession #: NP_079455.3
• NCBI GenBank Nucleotide #: NM_025179.3
• UniProt Accession #: O75051; Q6UX61; Q96GN9; Q9BRL1; Q9UIW1; A2RTX9; B2RMX7
• Molecular Weight: 61,649 Da
• NCBI Official Full Name: plexin-A2
• NCBI Official Synonym Full Names: plexin A2
• NCBI Official Symbol: PLXNA2
• NCBI Official Synonym Symbols: OCT; PLXN2
• NCBI Protein Information: plexin-A2
• UniProt Protein Name: Plexin-A2
• Protein Family: Plexin
• UniProt Gene Name: PLXNA2
• UniProt Synonym Gene Names: KIAA0463; OCT; PLXN2

NCBI Description

This gene encodes a member of the plexin-A family of semaphorin co-receptors. Semaphorins are a large family of secreted or membrane-bound proteins that mediate repulsive effects on axon pathfinding during nervous system development. A subset of semaphorins are recognized by plexin-A/neuropilin transmembrane receptor complexes, triggering a cellular signal transduction cascade that leads to axon repulsion. This plexin-A family member is thought to transduce signals from semaphorin-3A and -3C. [provided by RefSeq, Jul 2008]

Uniprot Description

PLXNA2: Coreceptor for SEMA3A and SEMA6A. Necessary for signaling by SEMA6A and class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down- stream signaling events in the cytoplasm. Homodimer. The PLXNA2 homodimer interacts with a SEMA6A homodimer, giving rise to a heterotetramer. Interacts directly with NRP1 and NRP2. Interacts with RND1. Detected in fetal brain. Belongs to the plexin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q32.2

Cellular Component: integral component of plasma membrane; plasma membrane

Molecular Function: identical protein binding; protein binding; semaphorin receptor activity

Biological Process: branchiomotor neuron axon guidance; centrosome localization; cerebellar granule cell precursor tangential migration; limb bud formation; neural tube development; pharyngeal system development; regulation of axon extension involved in axon guidance; regulation of cell migration; semaphorin-plexin signaling pathway; somitogenesis