PLTP recombinant protein

Recombinant Human PLTP Protein (His tag)

Cat. Num. AAA2545886

• Gene Names: PLTP; BPIFE; HDLCQ9
• Purity: > 80 % as determined by SDS-PAGE
• Synonyms: PLTP; Recombinant Human PLTP Protein (His tag); HDLCQ9; PLTP recombinant protein

• Host: Human Cells
• Purity/Purification: > 80 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 398

• Application Notes: The secreted recombinant human PLTP consists of 487 amino acids after removal of the signal peptide and has a predicted molecular mass of 54.5 kDa. As a result of glycosylation, the apparent molecular masss of rh PLTP is approximately 70-80 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Glu 18
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PLTP recombinant protein

Background: Phospholipid transfer protein, also known as Lipid transfer protein II and PLTP, is a secreted protein which belongs to the BPI/LBP/Plunc superfamily and BPI / LBP family. PLTP is nearly ubiquitously expressed in cells and tissues. PLTP converts HDL into larger and smaller particles. It may play a key role in extracellular phospholipid transport and modulation of hdl particles. High-density lipoproteins (HDL) play a major protective role against the development of coronary artery disease. PLTP is a main factor regulating the size and composition of HDL in the circulation and plays an important role in controlling plasma HDL levels. This is achieved via both the phospholipid transfer activity of PLTP and its capability to cause HDL conversion. PLTP is one of the key lipid transfer proteins in plasma and cerebrospinal fluid. It is involved in novel intracellular functions. PLTP is an important modulator of lipoprotein metabolism, including interparticle phospholipid transfer, remodeling of HDL, cholesterol and phospholipid efflux from peripheral tissues, and the production of hepatic VLDL. PLTP also plays an important role in inflammation and oxidative stress. Accordingly, PLTP has also been implicated in the development of atherosclerosis.

Description: A DNA sequence encoding the human PLTP isoform 1 (P55058-1) (Met 1-Val 493) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 339275807
• NCBI GeneID: 5360
• NCBI Accession #: NP_001229849.1
• NCBI GenBank Nucleotide #: NM_001242920.1
• UniProt Accession #: P55058; Q8WTT1; Q9BR07; Q9BSH8; A8K006; B4DDD5; B4DRB4; E1P5N8; E7EV16
• Molecular Weight: 44,847 Da
• NCBI Official Full Name: phospholipid transfer protein isoform c
• NCBI Official Synonym Full Names: phospholipid transfer protein
• NCBI Official Symbol: PLTP
• NCBI Official Synonym Symbols: BPIFE; HDLCQ9
• NCBI Protein Information: phospholipid transfer protein
• UniProt Protein Name: Phospholipid transfer protein
• Protein Family: Phospholipid transfer protein
• UniProt Gene Name: PLTP

NCBI Description

The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PLTP: Converts HDL into larger and smaller particles. May play a key role in extracellular phospholipid transport and modulation of hdl particles. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular region; extracellular space