Human Plastin-3 ELISA Kit | PLS3 elisa kit

Human Plastin-3 (PLS3) ELISA Kit

Cat. Num. AAA281055

• Gene Names: PLS3; BMND18; T-plastin
• Reactivity: Human
• Synonyms: Plastin-3; Human Plastin-3 (PLS3) ELISA Kit; PLS3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PLS3. No significant cross-reactivity or interference between Human PLS3 and analogues was observed.
• Sequence Length: 630

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PLS3 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLS3 in samples. An antibody specific for PLS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 209862851
• NCBI GeneID: 5358
• NCBI Accession #: NP_001129497.1
• NCBI GenBank Nucleotide #: NM_001136025.4
• UniProt Accession #: P13797; Q86YI6; A8K579; B1AQ09; B4DGB4; B7Z6M1
• Molecular Weight: 65,632 Da
• NCBI Official Full Name: plastin-3 isoform 1
• NCBI Official Synonym Full Names: plastin 3
• NCBI Official Symbol: PLS3
• NCBI Official Synonym Symbols: BMND18; T-plastin
• NCBI Protein Information: plastin-3
• UniProt Protein Name: Plastin-3
• Protein Family: Plastin
• UniProt Gene Name: PLS3

NCBI Description

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Uniprot Description

PLS3: Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: actin filament; actin filament bundle; cytoplasm; cytosol; plasma membrane

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin crosslink formation; actin filament bundle formation; actin filament network formation

Disease: Bone Mineral Density Quantitative Trait Locus 18