Human Plastin-1 ELISA Kit | PLS1 elisa kit

Human Plastin-1 (PLS1) ELISA Kit

Cat. Num. AAA280433

• Reactivity: Human
• Synonyms: Plastin-1; Human Plastin-1 (PLS1) ELISA Kit; PLS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PLS1. No significant cross-reactivity or interference between Human PLS1 and analogues was observed.
• Sequence Length: 629

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PLS1 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLS1 in samples. An antibody specific for PLS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 223718250
• NCBI GeneID: 5357
• NCBI Accession #: NP_001138791.1
• NCBI GenBank Nucleotide #: NM_001145319.1
• UniProt Accession #: Q14651; Q8NEG6; A8K2Q1; D3DNG3
• Molecular Weight: 70,253 Da
• NCBI Official Full Name: plastin-1
• NCBI Official Synonym Full Names: plastin 1
• NCBI Official Symbol: PLS1
• NCBI Protein Information: plastin-1
• UniProt Protein Name: Plastin-1
• Protein Family: Plastin
• UniProt Gene Name: PLS1
• UniProt Synonym Gene Names: I-plastin

NCBI Description

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Feb 2010]

Uniprot Description

PLS1: Actin-bundling protein in the absence of calcium.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: actin filament; actin filament bundle; brush border; cytoplasm

Molecular Function: actin filament binding; calcium ion binding; structural constituent of cytoskeleton

Biological Process: actin crosslink formation; actin filament bundle formation; actin filament network formation; D-glucose absorption; positive regulation of multicellular organism growth; regulation of microvillus length