Mitochondrial cardiolipin hydrolase (PLD6) Recombinant Protein | PLD6 recombinant protein

Recombinant Human Mitochondrial cardiolipin hydrolase (PLD6)

Cat. Num. AAA7135859

• Gene Names: PLD6; ZUC
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Mitochondrial cardiolipin hydrolase (PLD6); Recombinant Human Mitochondrial cardiolipin hydrolase (PLD6); Choline phosphatase 6 (Mitochondrial phospholipase); (MitoPLD); (Phosphatidylcholine-hydrolyzing phospholipase D6); (Phospholipase D6); (PLD 6); (Protein zucchini homolog); PLD6 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid or Lyophilized powder; If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.; If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, p
• Sequence Positions: 1-252. Full Length
• Sequence: MGRLSWQVAAAAAVGLALTLEALPWVLRWLRSRRRRPRREALFFPSQVTCTEALLRAPGAELAELPEGCPCGLPHGESALSRLLRALLAARASLDLCLFAFSSPQLGRAVQLLHQRGVRVRVVTDCDYMALNGSQIGLLRKAGIQVRHDQDPGYMHHKFAIVDKRVLITGSLNWTTQAIQNNRENVLITEDDEYVRLFLEEFERIWEQFNPTKYTFFPPKKSHGSCAPPVSRAGGRLLSWHRTCGTSSESQT

• Species: Human
• Tag: Tag-Free
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. ; Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C.; The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.; Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for PLD6 recombinant protein

Endonuclease that plays a critical role in PIWI-interacting RNA biogenesis during spermatogenesis. piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface. Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro, or very low. In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol. Has been shown to be a backbone-non-specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity. Produces 5' phosphate and 3' hydroxyl termini, suggesting it could directly participate in the processing of primary piRNA transcripts. Also acts as a regulator of mitochondrial shape through facilitating mitochondrial fusion.

Product Categories/Family for PLD6 recombinant protein

Epigenetics and Nuclear Signaling

References

"A common lipid links Mfn-mediated mitochondrial fusion and SNARE-regulated exocytosis."
Choi S.Y., Huang P., Jenkins G.M., Chan D.C., Schiller J., Frohman M.A.Nat. Cell Biol. 8:1255-1262(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 21410612
• NCBI GeneID: 201164
• UniProt Accession #: Q8N2A8
• Molecular Weight: 28.3kDa
• NCBI Official Full Name: Phospholipase D family, member 6
• NCBI Official Synonym Full Names: phospholipase D family member 6
• NCBI Official Symbol: PLD6
• NCBI Official Synonym Symbols: ZUC
• NCBI Protein Information: mitochondrial cardiolipin hydrolase
• UniProt Protein Name: Mitochondrial cardiolipin hydrolase
• Protein Family: Mitochondrial cardiolipin hydrolase
• UniProt Gene Name: PLD6
• UniProt Synonym Gene Names: MitoPLD; PLD 6
• UniProt Entry Name: PLD6_HUMAN