Secretory Phospholipase A2 Receptor 1 Recombinant Protein | PLA2R1 recombinant protein

Recombinant Human Secretory Phospholipase A2 Receptor 1, PLA2R1

Cat. Num. AAA184008

• Gene Names: PLA2R1; PLA2R; PLA2-R; PLA2IR; CLEC13C; PLA2G1R
• Applications: Western Blot
• Purity: Affinity chromatography. >95% as estimated by SDS-PAGE stained with Instant Blue Stain (Expedeon).
• Synonyms: Secretory Phospholipase A2 Receptor 1; Recombinant Human Secretory Phospholipase A2 Receptor 1; PLA2R1; 180 kDa secretory phospholipase A2 receptor; C-type lectin domain family 13 member C; M-type receptor; PLA2R1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: Affinity chromatography. >95% as estimated by SDS-PAGE stained with Instant Blue Stain (Expedeon).
• Form/Format: Liquid. PBS 20% Glycerol
• Sequence Positions: 21-1397aa
• Sequence: AEGVAAALTPERLLEWQDKGIFVIQSESLKKCIQAGKSVLTLENCKQANKHMLWKWVSNHGLFNIGGSGCLGLNFSAPEQPLSLYECDSTLVSLRWRCNRKMITGPLQYSVQVAHDNTVVASRKYIHKWISYGSGGGDICEYLHKDLHTIKGNTHGMPCMFPFQYNHQWHHECTREGREDDLLWCATTSRYERDEKWGFCPDPTSAEVGCDTIWEKDLNSHICYQFNLLSSLSWSEAHSSCQMQGGTLLSITDETEENFIREHMSSKTVEVWMGLNQLDEHAGWQWSDGTPLNYLNWSPEVNFEPFVEDHCGTFSSFMPSAWRSRDCESTLPYICKKYLNHIDHEIVEKDAWKYYATHCEPGWNPYNRNCYKLQKEEKTWHEALRSCQADNSALIDITSLAEVEFLVTLLGDENASETWIGLSSNKIPVSFEWSNDSSVIFTNWHTLEPHIFPNRSQLCVSAEQSEGHWKVKNCEERLFYICKKAGHVLSDAESGCQEGWERHGGFCYKIDTVLRSFDQASSGYYCPPALVTITNRFEQAFITSLISSVVKMKDSYFWIALQDQNDTGEYTWKPVGQKPEPVQYTHWNTHQPRYSGGCVAMRGRHPLGRWEVKHCRHFKAMSLCKQPVENQEKAEYEERWPFHPCYLDWESEPGLASCFKVFHSEKVLMKRTWREAEAFCEEFGAHLASFAHIEEENFVNELLHSKFNWTEERQFWIGFNKRNPLNAGSWEWSDRTPVVSSFLDNTYFGEDARNCAVYKANKTLLPLHCGSKREWICKIPRDVKPKIPFWYQYDVPWLFYQDAEYLFHTFASEWLNFEFVCSWLHSDLLTIHSAHEQEFIHSKIKALSKYGASWWIGLQEERANDEFRWRDGTPVIYQNWDTGRERTVNNQSQRCGFISSITGLWGSEECSVSMPSICKRKKVWLIEKKKDTPKQHGTCPKGWLYFNYKCLLLNIPKDPSSWKNWTHAQHFCAEEGGTLVAIESEVEQAFITMNLFGQTTSVWIGLQNDDYETWLNGKPVVYSNWSPFDIINIPSHNTTEVQKHIPLCALLSSNPNFHFTGKWYFEDCGKEGYGFVCEKMQDTSGHGVNTSDMYPMPNTLEYGNRTYKIINANMTWYAAIKTCLMHKAQLVSITDQYHQSFLTVVLNRLGYAHWIGLFTTDNGLNFDWSDGTKSSFTFWKDEESSLLGDCVFADSNGRWHSTACESFLQGAICHVPPETRQSEHPELCSETSIPWIKFKSNCYSFSTVLDSMSFEAAHEFCKKEGSNLLTIKDEAENAFLLEELFAFGSSVQMVWLNAQFDGNNETIKWFDGTPTDQSNWGIRKPDTDYFKPHHCVALRIPEGLWQLSPCQEKKGFICKMEADIHTAEALPEKGPSHSGSGHHHHHHHHHH

• Applicable Applications for PLA2R1 recombinant protein: Western Blot (WB), Immunoassay (IA), Functional Studies
• Source: Human
• Tag: 10xHis at the C-terminus
• Endotoxin: Less than 0.1ng/ug (1 IEU/ug), as measured by LAL method.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store at -20 degree or -70 degree C for at least 12 months; Shipping: Dry ice or frozen gel packs

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Related Product Information for PLA2R1 recombinant protein

PLA2R is a type I transmembrane glycoprotein of 180-200 kDa and is present in a widevariety of cells and tissues (1). Receptor for secretory phospholipase A2 (sPLA2) belongingto the mannose receptor family (2). It is composed of a large extracellular N-terminalportion, consisting of a N-terminal cystein-rich region, a fibronectin-like type II domain, atandem repeat of eight carbohydrate-recognition domains essential for ligand binding, andshort intracellular C-terminal region. Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B. Also able to bind to snake PA2-like toxins. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogenactivated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipidmediators, selective release of arachidonic acid in bone marrow-derived mast cells. Inneutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock (3). The soluble secretory phospholipase A2 receptorform is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B.
PLA2R is expressed on the surface of podocytes and represents a target auto antigen in 70% of patients with idiopathic membranous nephropathy (4). It has a test value in detecting and quantifying anti-PLA2R auto immunity.

References

I. Kanigicherla D., Gummadova J., McKenzie, EA., Roberts SA., Harris S., Nikam M., Poulton K., McWilliam L., Short CD., Venning M. and PE. Brenchley. Anti-PLA2 Antibodies Measured by ELISA Predict Long-Term Outcome in a Prevalent Population of Patients with Idiopathic Membranous Nephropathy. KidneyInternational. Vol. 83, No 5, pp 940-948. 2013.II. Fresquet M., Jowitt TA., Gummadova J., Collins R., O'Cualain R., McKenzie EA., Lennon R. and PE. Brenchley. Identification of a Major Epitope Recognised by PLA2R Autoantibodies in Primary Membranous Nephropathy. Journal of American Society of Nephrology. Vol. 26, No 2, pp 302-313. 2015.III. Fresquet M., Rhoden S.J., Jowitt TA., McKenzie EA., Roberts I., Lennon R. and PE. Brenchley. Autoantigens PLA2R1 and THSD7A in Membranous Nephropathy Share a Common Epitope Motif in the N-terminal Domain. Journal of Autoimmunity. 106, pp 1-12. 2020.

NCBI and Uniprot Product Information

• NCBI GI #: 119631798
• NCBI GeneID: 22925
• UniProt Accession #: Q13018; Q13019; Q15095; Q53R45; Q53RR7; B2RTU9; D3DPB1
• Molecular Weight: 168,600 Da
• NCBI Official Full Name: phospholipase A2 receptor 1, 180kDa, isoform CRA_b
• NCBI Official Synonym Full Names: phospholipase A2 receptor 1, 180kDa
• NCBI Official Symbol: PLA2R1
• NCBI Official Synonym Symbols: PLA2R; PLA2-R; PLA2IR; CLEC13C; PLA2G1R
• NCBI Protein Information: secretory phospholipase A2 receptor; M-type receptor; phospholipase A2 receptor 1, 180kD; C-type lectin domain family 13 member C; 180 kDa secretory phospholipase A2 receptor
• UniProt Protein Name: Secretory phospholipase A2 receptor
• Protein Family: Secretory phospholipase A2 receptor
• UniProt Gene Name: PLA2R1
• UniProt Synonym Gene Names: CLEC13C; PLA2-R; PLA2R; Soluble PLA2-R
• UniProt Entry Name: PLA2R_HUMAN

NCBI Description

This gene represents a phospholipase A2 receptor. The encoded protein likely exists as both a transmembrane form and a soluble form. The transmembrane receptor may play a role in clearance of phospholipase A2, thereby inhibiting its action. Polymorphisms at this locus have been associated with susceptibility to idiopathic membranous nephropathy. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]

Uniprot Description

PLA2R1: Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2- IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2- IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow- derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q23-q24

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; extracellular region; integral to membrane; receptor complex

Molecular Function: phospholipase binding; receptor activity; carbohydrate binding

Biological Process: receptor-mediated endocytosis; cytokine production; endocytosis; positive regulation of DNA damage response, signal transduction by p53 class mediator