Gene Sequencing (Extract)
Phospholipase A2, Group IIA (PLA2G2A) Recombinant Protein | PLA2G2A recombinant protein
Recombinant Phospholipase A2, Group IIA (PLA2G2A)
Gene Names
Pla2g2a; sPLA2
Reactivity
Rattus norvegicus (Rat)
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Phospholipase A2; Group IIA (PLA2G2A); Recombinant Phospholipase A2; PLA2G2A recombinant protein
Host
E.coli
Reactivity
Rattus norvegicus (Rat)
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 50ug/mL (varies by lot)
Sequence
SLLEFGQMI LFKTGKRADV SYGFYGCHCG VGGRGSPKDA TDWCCVTHDC CYNRLEKRGC GTKFLTYKFS YRGGQISCST NQDSCRKQLC QCDKAAAECF ARNKKSYSLK YQFYPNKFCK GKTPSC
Applicable Applications for PLA2G2A recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Ser22~Cys146
Tags
N-terminal His and GST Tag
Subcellular Location
Membrane, Secreted, Mitochondrion
Traits
Freeze-dried powder
Predicted isoelectric point
9.0
Usage
Reconstitute in ddH2O to a concentration of 0-0.5 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Gene Sequencing (Extract)
Gene Sequencing (Extract)
SDS-PAGE
SDS-PAGE
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted MW: 9.0
Observed MW:44 kDa as determined by SDS-PAGE reducing conditions.
Observed MW:44 kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
phospholipase A2, membrane associated
NCBI Official Synonym Full Names
phospholipase A2, group IIA (platelets, synovial fluid)
NCBI Official Symbol
Pla2g2a
NCBI Official Synonym Symbols
sPLA2
NCBI Protein Information
phospholipase A2, membrane associated; GIIC sPLA2; platelet phospholipase A2; group IIA phospholipase A2; eted enzyme type IIA phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A
UniProt Protein Name
Phospholipase A2, membrane associated
Protein Family
UniProt Gene Name
Pla2g2a
UniProt Entry Name
PA2GA_RAT
NCBI Description
hydrolyzes acyl group at the sn-2 position of phospholipids, forming non-esterified fatty acids and lysophospholipids; plays a critical role in inflammation; induces proliferation of smooth muscle cells [RGD, Feb 2006]
Uniprot Description
Function: Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity: Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor: Binds 1 calcium ion per subunit
By similarity.
Subcellular location: Membrane; Peripheral membrane protein.
Miscellaneous: Group II phospholipase A2 is found in many cells and also extracellularly. The membrane-bound and secreted forms are identical and are encoded by a single gene.
Sequence similarities: Belongs to the phospholipase A2 family.
Research Articles on PLA2G2A
Similar Products
Product Notes
The PLA2G2A pla2g2a (Catalog #AAA2018441) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Phospholipase A2, Group IIA (PLA2G2A) reacts with Rattus norvegicus (Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospholipase A2, Group IIA (PLA2G2A) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the PLA2G2A pla2g2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SLLEFGQMI LFKTGKRADV SYGFYGCHCG VGGRGSPKDA TDWCCVTHDC CYNRLEKRGC GTKFLTYKFS YRGGQISCST NQDSCRKQLC QCDKAAAECF ARNKKSYSLK YQFYPNKFCK GKTPSC. It is sometimes possible for the material contained within the vial of "Phospholipase A2, Group IIA (PLA2G2A), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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