Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
PKN1/PRK1 recombinant protein
PKN1/PRK1, Active
Gene Names
PKN1; DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
Applications
Western Blot
Synonyms
PKN1/PRK1; Active; PRK1; DBK; PKN1; PKN; MGC46204; PAK1; PRKCL1; PKC-L1; PKN1/PRK1 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for PKN1/PRK1 recombinant protein
Kinase Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for PKN1/PRK1 recombinant protein
Recombinant human PKN1/PRK1 (289-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The MSK2 gene accession number is BC040061.
PKN1/PRK1 belongs to the protein kinase C superfamily which is activated by Rho family of small G proteins. PKN1/PRK1 is known to mediate the Rho-dependent signaling pathway and it can be activated by phospholipids and by limited proteolysis (1). PDPK1/PDK may also mediate insulin signals to the actin cytoskeleton and the proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggest its role in signal transduction related to apoptosis. PKN1/PRK signaling stimulates AR activity in the presence of adrenal androgens and in the presence of an AR antagonist (2).
PKN1/PRK1 belongs to the protein kinase C superfamily which is activated by Rho family of small G proteins. PKN1/PRK1 is known to mediate the Rho-dependent signaling pathway and it can be activated by phospholipids and by limited proteolysis (1). PDPK1/PDK may also mediate insulin signals to the actin cytoskeleton and the proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggest its role in signal transduction related to apoptosis. PKN1/PRK signaling stimulates AR activity in the presence of adrenal androgens and in the presence of an AR antagonist (2).
Product Categories/Family for PKN1/PRK1 recombinant protein
References
1. Amano, M.et.al: Identification of a putative target for rho as the serine-threonine kinase protein kinase N. Science 271: 648-651, 1996.
2. Metzger, E. et.al: A novel inducible transactivation domain in the androgen receptor: implications for PRK in prostate cancer. EMBO J. 22: 270-280, 2003.
2. Metzger, E. et.al: A novel inducible transactivation domain in the androgen receptor: implications for PRK in prostate cancer. EMBO J. 22: 270-280, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
66,060 Da
NCBI Official Full Name
Homo sapiens protein kinase N1, mRNA
NCBI Official Synonym Full Names
protein kinase N1
NCBI Official Symbol
PKN1
NCBI Official Synonym Symbols
DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
NCBI Protein Information
serine/threonine-protein kinase N1
NCBI Description
The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Research Articles on PKN1/PRK1
Similar Products
Product Notes
The PKN1/PRK1 (Catalog #AAA517461) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PKN1/PRK1 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the PKN1/PRK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKN1/PRK1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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