Human Polycystin-2 ELISA Kit | PKD2 elisa kit
Human Polycystin-2 (PKD2) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PKD2 in Human serum, plasma. An antibody specific for PKD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PKD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PKD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PKD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the polycystin protein family. The encoded protein is a multi-pass membrane protein that functions as a calcium permeable cation channel, and is involved in calcium transport and calcium signaling in renal epithelial cells. This protein interacts with polycystin 1, and they may be partners in a common signaling cascade involved in tubular morphogenesis. Mutations in this gene are associated with autosomal dominant polycystic kidney disease type 2. [provided by RefSeq, Mar 2011]
Uniprot Description
PKD2: Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis. Acts as a regulator of cilium length, together with PKD1. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. Functions as a calcium permeable cation channel. Defects in PKD2 are the cause of polycystic kidney disease autosomal dominant type 2 (ADPKD2). ADPKD2 is a disorder characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. It represents approximately 15% of the cases of autosomal dominant polycystic kidney disease. ADPKD2 is clinically milder than ADPKD1 but it has a deleterious impact on overall life expectancy. Belongs to the polycystin family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Channel, cation; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 4q22.1
Cellular Component: basal cortex; basal plasma membrane; cilium; cytoplasm; cytoplasmic vesicle membrane; endoplasmic reticulum; endoplasmic reticulum membrane; extracellular exosome; filamentous actin; integral component of cytoplasmic side of endoplasmic reticulum membrane; integral component of lumenal side of endoplasmic reticulum membrane; integral component of plasma membrane; intercellular junction; lamellipodium; plasma membrane
Molecular Function: actinin binding; ATPase binding; calcium ion binding; calcium-induced calcium release activity; cytoskeletal protein binding; HLH domain binding; identical protein binding; ion channel binding; muscle alpha-actinin binding; outward rectifier potassium channel activity; phosphoprotein binding; potassium channel activity; protein binding; protein homodimerization activity; receptor binding; voltage-gated calcium channel activity; voltage-gated cation channel activity; voltage-gated ion channel activity; voltage-gated potassium channel activity; voltage-gated sodium channel activity
Biological Process: branching involved in ureteric bud morphogenesis; calcium ion transport; cell cycle arrest; cellular response to calcium ion; cellular response to cAMP; cellular response to fluid shear stress; cellular response to hydrostatic pressure; cellular response to osmotic stress; cellular response to reactive oxygen species; centrosome duplication; cytoplasmic sequestering of transcription factor; detection of mechanical stimulus; detection of nodal flow; determination of left/right symmetry; determination of liver left/right asymmetry; embryonic placenta development; heart development; heart looping; JAK-STAT cascade; liver development; mesonephric duct development; mesonephric tubule development; metanephric ascending thin limb development; metanephric cortex development; metanephric cortical collecting duct development; metanephric distal tubule development; metanephric mesenchyme development; metanephric S-shaped body morphogenesis; metanephric smooth muscle tissue development; negative regulation of cell proliferation; negative regulation of G1/S transition of mitotic cell cycle; neural tube development; positive regulation of cell cycle arrest; positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle; positive regulation of inositol-1,4,5-triphosphate receptor activity; positive regulation of nitric oxide biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; potassium ion transmembrane transport; protein homotetramerization; regulation of calcium ion import; regulation of cAMP metabolic process; regulation of cell proliferation; release of sequestered calcium ion into cytosol; spinal cord development
Disease: Polycystic Kidney Disease 2
Research Articles on PKD2
Similar Products
Product Notes
The Human PKD2 pkd2 (Catalog #AAA282949) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282949 ELISA Kit recognizes Human PKD2. It is sometimes possible for the material contained within the vial of "Polycystin-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
