Canine Protein Kinase C Delta ELISA Kit | PKCd elisa kit

Canine Protein Kinase C Delta ELISA Kit

Cat. Num. AAA746597

• Gene Names: PRKCD; MAY1; PKCD; CVID9; nPKC-delta
• Reactivity: Canine
• Synonyms: Protein Kinase C Delta; Canine Protein Kinase C Delta ELISA Kit; PKCd elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKCd elisa kit

Intended Uses: This PKCD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine PKCD. This ELISA kit for research use only!

Principle of the Assay: PKCD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKCD antibody and an PKCD-HRP conjugate. The assay sample and buffer are incubated together with PKCD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKCD concentration since PKCD from samples and PKCD-HRP conjugate compete for the anti-PKCD antibody binding site. Since the number of sites is limited, as more sites are occupied by PKCD from the sample, fewer sites are left to bind PKCD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKCD concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PKCd elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 509050
• NCBI GeneID: 5580
• UniProt Accession #: Q05655; Q15144; Q86XJ6; B0KZ81; B2R834
• Molecular Weight: 80,953 Da
• NCBI Official Full Name: protein kinase C delta, partial
• NCBI Official Synonym Full Names: protein kinase C, delta
• NCBI Official Symbol: PRKCD
• NCBI Official Synonym Symbols: MAY1; PKCD; CVID9; nPKC-delta
• NCBI Protein Information: protein kinase C delta type; protein kinase C delta VIII; tyrosine-protein kinase PRKCD
• UniProt Protein Name: Protein kinase C delta type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCD
• UniProt Synonym Gene Names: SDK1
• UniProt Entry Name: KPCD_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCD: an AGC kinase of the PKC family. A novel PKC: Ca2 independent but still regulated by PS, DAG and phorbol esters. Caspase-3 appears to liberate a sphingosine-activated catalytic fragment that has altered regulatory mechanisms and substrate specificities.

Protein type: Nuclear receptor co-regulator; EC 2.7.11.13; Tumor suppressor; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; PKC family; Delta subfamily

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; plasma membrane; intercellular junction; cytosol; nucleus

Molecular Function: calcium-independent protein kinase C activity; protein serine/threonine kinase activity; protein binding; protein kinase C activity; enzyme binding; metal ion binding; insulin receptor substrate binding; enzyme activator activity; non-membrane spanning protein tyrosine kinase activity; ATP binding

Biological Process: B cell proliferation; negative regulation of MAP kinase activity; interleukin-12 production; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; apoptosis; negative regulation of insulin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; positive regulation of protein amino acid dephosphorylation; negative regulation of filopodium formation; positive regulation of superoxide release; regulation of receptor activity; negative regulation of protein binding; cell structure disassembly during apoptosis; epidermal growth factor receptor signaling pathway; negative regulation of peptidyl-tyrosine phosphorylation; platelet activation; fibroblast growth factor receptor signaling pathway; neutrophil activation; protein stabilization; negative regulation of actin filament polymerization; cytokine and chemokine mediated signaling pathway; peptidyl-threonine phosphorylation; cell cycle; phospholipase C activation; negative regulation of inflammatory response; defense response to bacterium; interleukin-10 production; innate immune response; gene expression; immunoglobulin mediated immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation; induction of apoptosis by oxidative stress

Disease: Autoimmune Lymphoproliferative Syndrome, Type Iii