Rat Protein Kinase B ELISA Kit | PKB elisa kit

Rat Protein Kinase B ELISA Kit

Cat. Num. AAA11112

• Gene Names: PTK2B; PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK
• Reactivity: Rat
• Synonyms: Protein Kinase B; Rat Protein Kinase B ELISA Kit; PKB elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes recombinant and natural Rat PKB, no significant cross-reactivity or interference was observed.
• Sequence Length: 967

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Detection Range: 25 U/L - 800 U/L.
• Sensitivity: 2.0 U/L.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Rat PKB in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Rat PKB concentration. The concentration of Rat PKB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) are less than 15%.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKB elisa kit

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 27886588
• NCBI GeneID: 2185
• NCBI Accession #: NP_775267.1
• NCBI GenBank Nucleotide #: NM_173175.2
• UniProt Accession #: Q14289; Q13475; Q14290; Q16709; Q6PID4; D3DST0
• Molecular Weight: 115,875 Da
• NCBI Official Full Name: protein-tyrosine kinase 2-beta isoform b
• NCBI Official Synonym Full Names: protein tyrosine kinase 2 beta
• NCBI Official Symbol: PTK2B
• NCBI Official Synonym Symbols: PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK
• NCBI Protein Information: protein-tyrosine kinase 2-beta; FADK 2; CAK-beta; protein kinase B; focal adhesion kinase 2; cell adhesion kinase beta; proline-rich tyrosine kinase 2; calcium-dependent tyrosine kinase; PTK2B protein tyrosine kinase 2 beta; related adhesion focal tyrosine kinase; calcium-regulated non-receptor proline-rich tyrosine kinase
• UniProt Protein Name: Protein-tyrosine kinase 2-beta
• Protein Family: Protein kinase
• UniProt Gene Name: PTK2B
• UniProt Synonym Gene Names: FAK2; PYK2; RAFTK; CADTK; CAK-beta; CAKB; FADK 2; RAFTK
• UniProt Entry Name: FAK2_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Pyk2: a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. It plays an important role in cell motility such as spreading and migration. Two alternatively spliced isoforms have been described.

Protein type: Nuclear receptor co-regulator; Kinase, protein; Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Fak family

Chromosomal Location of Human Ortholog: 8p21.1

Cellular Component: focal adhesion; dendrite; postsynaptic density; cell cortex; cytosol; N-methyl-D-aspartate selective glutamate receptor complex; lipid raft; nucleoplasm; extrinsic to internal side of plasma membrane; cytoskeleton; growth cone; cell soma; axon; perinuclear region of cytoplasm; lamellipodium; cytoplasm; nucleus

Molecular Function: signal transducer activity; protein binding; calmodulin-dependent protein kinase activity; N-methyl-D-aspartate selective glutamate receptor activity; protein-tyrosine kinase activity; 3-phosphoinositide-dependent protein kinase binding; protein complex binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: focal adhesion formation; positive regulation of JNK activity; regulation of nitric oxide biosynthetic process; regulation of cGMP biosynthetic process; protein amino acid phosphorylation; glial cell proliferation; negative regulation of bone mineralization; regulation of inositol trisphosphate biosynthetic process; regulation of cell shape; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; response to glucose stimulus; protein complex assembly; negative regulation of neuron apoptosis; neurite development; bone resorption; response to drug; negative regulation of myeloid cell differentiation; positive regulation of nitric-oxide synthase activity; response to osmotic stress; positive regulation of cell growth; marginal zone B cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; response to ethanol; ionotropic glutamate receptor signaling pathway; response to mechanical stimulus; response to calcium ion; negative regulation of apoptosis; activation of JAK protein; response to cAMP; peptidyl-tyrosine phosphorylation; positive regulation of translation; blood vessel endothelial cell migration; apoptosis; response to lithium ion; negative regulation of potassium ion transport; protein amino acid autophosphorylation; response to hormone stimulus; positive regulation of JNK cascade; regulation of calcium-mediated signaling; signal transduction; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell proliferation; positive regulation of cell proliferation; response to stress; angiogenesis; positive regulation of cell-matrix adhesion; epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; regulation of cell adhesion; MAPKKK cascade; signal complex assembly; positive regulation of phosphoinositide 3-kinase activity; response to cocaine; oocyte maturation; regulation of release of sequestered calcium ion into cytosol; positive regulation of actin filament polymerization; response to hydrogen peroxide; positive regulation of protein kinase activity; stress fiber formation; response to hypoxia; innate immune response; cellular defense response; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration