Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform ELISA Kit | PIK3CD elisa kit

Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform ELISA Kit

Cat. Num. AAA2883939

• Reactivity: Human
• Synonyms: Phosphatidylinositol-4; 5-bisphosphate 3-kinase catalytic subunit delta isoform; Human Phosphatidylinositol-4; 5-bisphosphate 3-kinase catalytic subunit delta isoform ELISA Kit; PI3-kinase subunit delta; PI3K-delta; PI3Kdelta; PtdIns-3-kinase subunit delta; PIK3CD elisa kit

• Reactivity: Human
• Sequence Length: 1044

• Assay Type: Sandwich
• Detection Range: 0.312-20 ng/mL
• Sensitivity: 0.35ng/ml
• Intra-Assay CV: <=5.4%
• Inter-Assay CV: <=9.7%
• Recovery: 99%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 156564405
• NCBI GeneID: 5293
• NCBI Accession #: NP_005017.3
• NCBI GenBank Nucleotide #: NM_005026.3
• UniProt Accession #: O00329; O15445; Q5SR49; A6NCG0; G1FFP1
• Molecular Weight: 33,107 Da
• NCBI Official Full Name: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• UniProt Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• Protein Family: Phosphatidylinositol 4,5-bisphosphate 3-kinase
• UniProt Gene Name: PIK3CD
• UniProt Synonym Gene Names: PI3-kinase subunit delta; PI3K-delta; PI3Kdelta; PtdIns-3-kinase subunit delta; PtdIns-3-kinase subunit p110-delta; p110delta
• UniProt Entry Name: PK3CD_HUMAN

Uniprot Description

PIK3CD: Phosphoinositide-3-kinase (PI3K) that phosphorylates PftdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Mediates immune responses. Plays a role in B-cell development, proliferation, migration, and function. Required for B-cell receptor (BCR) signaling. Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation. Promotes cytokine production in response to TLR4 and TLR9. Required for antibody class switch mediated by TLR9. Involved in the antigen presentation function of B-cells. Involved in B-cell chemotaxis in response to CXCL13 and sphingosine 1-phosphate (S1P). Required for proliferation, signaling and cytokine production of naive, effector and memory T-cells. Required for T-cell receptor (TCR) signaling. Mediates TCR signaling events at the immune synapse. Activation by TCR leads to antigen-dependent memory T-cell migration and retention to antigenic tissues. Together with PIK3CG participates in T-cell development. Contributes to T-helper cell expansion and differentiation. Required for T-cell migration mediated by homing receptors SELL/CD62L, CCR7 and S1PR1 and antigen dependent recruitment of T-cells. Together with PIK3CG is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in NK cell receptor activation. Have a role in NK cell maturation and cytokine production. Together with PIK3CG is involved in neutrophil chemotaxis and extravasation. Together with PIK3CG participates in neutrophil respiratory burst. Have important roles in mast-cell development and mast cell mediated allergic response. Involved in stem cell factor (SCF)-mediated proliferation, adhesion and migration. Required for allergen-IgE-induced degranulation and cytokine release. The lipid kinase activity is required for its biological function. Isoform 2 may be involved in stabilizing total RAS levels, resulting in increased ERK phosphorylation and increased PI3K activity. Belongs to the PI3/PI4-kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, lipid; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.153

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: cytosol; mast cell granule; phosphoinositide 3-kinase complex; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity; protein binding

Biological Process: adaptive immune response; B cell activation; B cell homeostasis; B cell receptor signaling pathway; cytokine production; defense response to fungus; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; inflammatory response; innate immune response; mast cell chemotaxis; mast cell degranulation; natural killer cell activation; natural killer cell differentiation; nerve growth factor receptor signaling pathway; neutrophil chemotaxis; phosphatidylinositol biosynthetic process; phosphoinositide 3-kinase cascade; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; phospholipid metabolic process; platelet activation; positive regulation of cell migration; protein amino acid phosphorylation; respiratory burst during defense response; signal transduction; T cell activation; T cell differentiation; T cell receptor signaling pathway

Disease: Immunodeficiency 14