Human Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta ELISA Kit | PIK3C2B elisa kit

Human Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta (PIK3C2B) ELISA Kit

Cat. Num. AAA282629

• Gene Names: PIK3C2B; C2-PI3K
• Reactivity: Human
• Synonyms: Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta; Human Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta (PIK3C2B) ELISA Kit; PIK3C2B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PIK3C2B. No significant cross-reactivity or interference between Human PIK3C2B and analogues was observed.
• Sequence Length: 1634

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.31 ng/mL - 20 ng/mL
• Sensitivity: 0.1 ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PIK3C2B elisa kit

Intended Uses: For the quantitative detection of Human Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta (PIK3C2B) concentration in serum, plasma and other biological fluids.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PIK3C2B in Human serum, plasma. An antibody specific for PIK3C2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIK3C2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3C2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3C2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 194097347
• NCBI GeneID: 5287
• NCBI Accession #: NP_002637.3
• NCBI GenBank Nucleotide #: NM_002646.3
• UniProt Accession #: O00750; O95666; Q5SW99
• Molecular Weight: 184,768 Da
• NCBI Official Full Name: phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta
• NCBI Official Symbol: PIK3C2B
• NCBI Official Synonym Symbols: C2-PI3K
• NCBI Protein Information: phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta
• UniProt Protein Name: Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta
• Protein Family: Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing
• UniProt Gene Name: PIK3C2B
• UniProt Synonym Gene Names: PI3K-C2-beta; PtdIns-3-kinase C2 subunit beta

NCBI Description

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner. [provided by RefSeq, Jul 2008]

Uniprot Description

PIK3C2B: Phosphorylates PtdIns and PtdIns4P with a preference for PtdIns. Does not phosphorylate PtdIns(4,5)P2. May be involved in EGF and PDGF signaling cascades. Belongs to the PI3/PI4-kinase family.

Protein type: Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.154; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: cytoplasm; cytosol; endocytic vesicle; endoplasmic reticulum; nucleoplasm; phosphoinositide 3-kinase complex; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; lipid kinase activity; phosphatidylinositol binding; phosphatidylinositol-4-phosphate 3-kinase activity; protein binding

Biological Process: autophagosome organization; cell migration; cellular response to starvation; phosphatidylinositol biosynthetic process; phosphatidylinositol phosphorylation; phosphoinositide 3-kinase cascade; protein kinase B signaling