Pig Renin (REN) ELISA Kit | REN elisa kit
Pig Renin (REN) ELISA Kit
Gene Names
RENBP; AGE; RnBP
Reactivity
Pig
Synonyms
Renin (REN); Pig Renin (REN) ELISA Kit; FLJ10761; HNFJ2; angiotensin-forming enzyme|angiotensinogenase|renin precursor; renal; REN elisa kit
Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig REN. No significant cross-reactivity or interference between Pig REN and analogues was observed.
Assay Type
Sandwich
Assay
Serum, Plasma, Other biological fluids
Detection Range
62.5-4000pg/mL
Sensitivity
1.56pg/mL
Analysis Method
Quantitive
Detection Wavelength
450nm
Sample Volume
1-200uL
Assay Duration
1-4.5h
Precision
Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Test Principle
This assay employs a two-site sandwich ELISA to quantitate REN in samples. An antibody specific for REN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Preparation and Storage
Store at 2-8 degree C. Please refer to Instruction Manual.
Related Product Information for REN elisa kit
Renin is a member of the aspartyl proteinase family produced largely in part by the juxtaglomerular cells in the kidney.Renin differs from the other members of this class by having a pH optimum near neutral pH region with native substrates instead of 2.0 to 3.4 This more neutral pH optimum allows it to be functional in the plasma.
Renin also has very high selectivity for substrate due to quite long peptide recognition on either side of the peptide bond undergoing cleavage. An octapeptide substrate was the minimum length to be cleaved by renin. Renin plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of renin.
Renin also has very high selectivity for substrate due to quite long peptide recognition on either side of the peptide bond undergoing cleavage. An octapeptide substrate was the minimum length to be cleaved by renin. Renin plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of renin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,380 Da
NCBI Official Full Name
N-acylglucosamine 2-epimerase
NCBI Official Symbol
RENBP
NCBI Official Synonym Symbols
AGE; RnBP
NCBI Protein Information
N-acylglucosamine 2-epimerase; glcNAc 2-epimerase; renin-binding protein; N-acetyl-D-glucosamine 2-epimerase
UniProt Protein Name
N-acylglucosamine 2-epimerase
Protein Family
UniProt Gene Name
RENBP
UniProt Synonym Gene Names
AGE; RnBP
UniProt Entry Name
RENBP_BOVIN
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Product Notes
The Pig REN renbp (Catalog #AAA2802068) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2802068 ELISA Kit recognizes Pig REN. It is sometimes possible for the material contained within the vial of "Renin (REN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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