Pig Fatty Acid Binding Protein 2, Intestinal(FABP2) ELISA Kit | FABP2 elisa kit
Pig Fatty Acid Binding Protein 2, Intestinal(FABP2) ELISA Kit
Gene Names
FABP2; FABPI; I-FABP
Reactivity
Pig
Synonyms
Fatty Acid Binding Protein 2; Intestinal(FABP2); Pig Fatty Acid Binding Protein 2; Intestinal(FABP2) ELISA Kit; FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein; FABP2 elisa kit
Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of FABP1. No significant cross-reactivity or interference between FABP1 and analogues was observed.
Sequence Length
132
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.109ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FABP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FABP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FABP2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FABP1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FABP1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FABP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FABP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FABP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FABP1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FABP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FABP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FABP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for FABP2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
15,207 Da
NCBI Official Full Name
fatty acid binding protein 2, intestinal
NCBI Official Synonym Full Names
fatty acid binding protein 2
NCBI Official Symbol
FABP2
NCBI Official Synonym Symbols
FABPI; I-FABP
NCBI Protein Information
fatty acid-binding protein, intestinal
UniProt Protein Name
Fatty acid-binding protein, intestinal
Protein Family
UniProt Gene Name
FABP2
UniProt Synonym Gene Names
FABPI; I-FABP
NCBI Description
The protein encoded by this gene is an intracellular fatty acid-binding protein that participates in the uptake, intracellular metabolism, and transport of long-chain fatty acids. The encoded protein is also involved in the modulation of cell growth and proliferation. This protein binds saturated long-chain fatty acids with high affinity, and may act as a lipid sensor to maintain energy homeostasis. [provided by RefSeq, Aug 2017]
Uniprot Description
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
