Transcription factor PIF1 (PIF1) Recombinant Protein | PIF1 recombinant protein

Recombinant Arabidopsis thaliana Transcription factor PIF1 (PIF1)

Cat. Num. AAA1345597

• Gene Names: PIL5; PHY-INTERACTING FACTOR 1; phytochrome interacting factor 3-like 5; PIF1; T2G17.2; T2G17_2
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Transcription factor PIF1 (PIF1); Recombinant Arabidopsis thaliana Transcription factor PIF1 (PIF1); PIF1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-478, Full length protein
• Sequence: MHHFVPDFDTDDDYVNNHNSSLNHLPRKSITTMGEDDDLMELLWQNGQVVVQNQRLHTKKPSSSPPKLLPSMDPQQQPSSDQNLFIQEDEMTSWLHYPLRDDDFCSDLLFSAAPTATATATVSQVTAARPPVSSTNESRPPVRNFMNFSRLRGDFNNGRGGESGPLLSKAVVRESTQVSPSATPSAAASESGLTRRTDGTDSSAVAGGGAYNRKGKAVAMTAPAIEITGTSSSVVSKSEIEPEKTNVDDRKRKEREATTTDETESRSEETKQARVSTTSTKRSRAAEVHNLSERKRRDRINERMKALQELIPRCNKSDKASMLDEAIEYMKSLQLQIQMMSMGCGMMPMMYPGMQQYMPHMAMGMGMNQPIPPPSFMPFPNMLAAQRPLPTQTHMAGSGPQYPVHASDPSRVFVPNQQYDPTSGQPQYPAGYTDPYQQFRGLHPTQPPQFQNQATSYPSSSRVSSSKESEDHGNHTTG
• Sequence Length: 478

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 334184322
• NCBI GeneID: 816538
• NCBI Accession #: NP_001189559.1
• NCBI GenBank Nucleotide #: NM_001202630.2
• UniProt Accession #: Q8GZM7; Q0WQ83; Q3EBY0; Q9SL63
• Molecular Weight: 44,715 Da
• NCBI Official Full Name: phytochrome interacting factor 3-like 5
• NCBI Official Symbol: PIL5
• NCBI Official Synonym Symbols: PHY-INTERACTING FACTOR 1; phytochrome interacting factor 3-like 5; PIF1; T2G17.2; T2G17_2
• NCBI Protein Information: phytochrome interacting factor 3-like 5
• UniProt Protein Name: Transcription factor PIF1
• Protein Family: Transcription factor
• UniProt Gene Name: PIF1
• UniProt Synonym Gene Names: BHLH15; EN101; PIL5; AtbHLH15; bHLH 15

NCBI Description

Encodes a novel Myc-related bHLH transcription factor that has transcriptional activation activity in the dark. It is a key negative regulator of phytochrome-mediated seed germination and acts by inhibiting chlorophyll biosynthesis, light-mediated suppression of hypocotyl elongation and far-red light-mediated suppression of seed germination, and promoting negative gravitropism in hypocotyls. Light reduces this activity in a phy-dependent manner. The protein preferentially interacts with the Pfr forms of Phytochrome A (PhyA) and Phytochrome B (PhyB), is physically associated with APRR1/TOC1 and is degraded in red (R) and far-red (FR) light through the ubiquitin (ub)-26S proteasome pathway to optimize photomorphogenic development in Arabidopsis. It also negatively regulates GA3 oxidase expression.

Uniprot Description

Transcription activator. Regulates negatively chlorophyll biosynthesis and seed germination in the dark, and lightinduced degradation of PIF1 relieves this negative regulation to promote photomorphogenesis. Binds to the G-box motif (5'-CACGTG-3') found in many light-regulated promoters. Promotes the expression of SOM, and thus modulates responses to abscisic acid (ABA) and gibberellic acid (GA).