Phosphatase 2A Activator Recombinant Protein | PPP2R4 recombinant protein
Recombinant Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Phosphatase 2A Activator; Recombinant Protein Phosphatase 2A Activator; Regulatory Subunit 4 (PPP2R4); PPP2R4 recombinant protein
Host
Host: E Coli
Source: Prokaryotic expression
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MAEGERQPPP DSSEETPSAT PTFIIPKKEI HTVPDMGKWK RSQAYADYIGFILTLNEGVK GKKLTFDYKV SEAIEKLVAL LDTLDRWIDE TPPVDQPSRFGNKAYRTWYA KLDQEAENLV ATVIPTNLAA AVPEVAVYLK EAVGNSTRIDYGTGHEAAFA AFLCCLCKIG VLRVDDQVAI VFKVFDRYLE VMRKLQKTYRMEPAGSQGVW GLDDFQFLPF IWGSSQLIDH PHLEPRHFVD EKAVSENHKDYMFLQCILFI TEMKTGPFAE HSNQLWNISA VPSWSKVNQG LIRMYKAECLEKFPVIQHFK FGSLLPIHPV TSG
MAEGERQPPP DSSEETPSAT PTFIIPKKEI HTVPDMGKWK RSQAYADYIGFILTLNEGVK GKKLTFDYKV SEAIEKLVAL LDTLDRWIDE TPPVDQPSRFGNKAYRTWYA KLDQEAENLV ATVIPTNLAA AVPEVAVYLK EAVGNSTRIDYGTGHEAAFA AFLCCLCKIG VLRVDDQVAI VFKVFDRYLE VMRKLQKTYRMEPAGSQGVW GLDDFQFLPF IWGSSQLIDH PHLEPRHFVD EKAVSENHKDYMFLQCILFI TEMKTGPFAE HSNQLWNISA VPSWSKVNQG LIRMYKAECLEKFPVIQHFK FGSLLPIHPV TSG
Sequence Length
323
Applicable Applications for PPP2R4 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
40.3kDa
Accurate Molecular Mass (KD)
40kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and T7-tag
Organism Species
Rattus norvegicus (Rat)
Fragment
Met1~Gly323
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
PPP2R4, partial
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Product Notes
The PPP2R4 (Catalog #AAA2031776) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phosphatase 2A Activator can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the PPP2R4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.MAEGERQPP P DSSEETPSAT PTFIIPKKEI HTVPDMGKWK RSQAYADYIG FILTLNEGVK GKKLTFDYKV SEAIEKLVAL LDTLDRWIDE TPPVDQPSRF GNKAYRTWYA KLDQEAENLV ATVIPTNLAA AVPEVAVYLK EAVGNSTRID YGTGHEAAFA AFLCCLCKIG VLRVDDQVAI VFKVFDRYLE VMRKLQKTYR MEPAGSQGVW GLDDFQFLPF IWGSSQLIDH PHLEPRHFVD EKAVSENHKD YMFLQCILFI TEMKTGPFAE HSNQLWNISA VPSWSKVNQG LIRMYKAECL EKFPVIQHFK FGSLLPIHPV TSG. It is sometimes possible for the material contained within the vial of "Phosphatase 2A Activator, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
