Phosphatidylglycerophosphatase B (pgpB) Recombinant Protein | pgpB recombinant protein
Recombinant Escherichia coli Phosphatidylglycerophosphatase B (pgpB)
NCBI and Uniprot Product Information
NCBI Description
The pgpB gene is nonessential, but is synthetically lethal with a bacA ybjG double mutant, due to its UppP activity. ybjG, pgpB, and lpxT are paralogs. [More information is available at EcoGene: EG10705]. PgpB is one of three phosphatidylglycerophosphatase (PGP-ase) activities in Escherichia coli. [More information is available at EcoCyc: EG10705].
Uniprot Description
Function: Catalyzes the dephosphorylation of diacylglycerol diphosphate (DGPP) to phosphatidate (PA) and the subsequent dephosphorylation of PA to diacylglycerol (DAG). Also has undecaprenyl pyrophosphate phosphatase activity, required for the biosynthesis of the lipid carrier undecaprenyl phosphate. Can also use lysophosphatidic acid (LPA) and phosphatidylglycerophosphate as substrates. The pattern of activities varies according to subcellular location, PGP phosphatase activity is higher in the cytoplasmic membrane, whereas PA and LPA phosphatase activities are higher in the outer membrane. Activity is independent of a divalent cation ion and insensitive to inhibition by N-ethylmaleimide. Ref.5 Ref.6 Ref.8 Ref.9
Catalytic activity: Phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate. Ref.5 Ref.81,2-diacyl-sn-glycerol 3-diphosphate + H2O = 1,2-diacyl-sn-glycerol 3-phosphate + phosphate. Ref.5 Ref.8A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate. Ref.5 Ref.8Ditrans,octacis-undecaprenyl diphosphate + H2O = ditrans,octacis-undecaprenyl phosphate + phosphate. Ref.5 Ref.8
Enzyme regulation: Inhibited by Mn2+ ions.
Pathway: Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 2/2.
Subcellular location: Cell inner membrane; Multi-pass membrane protein. Cell outer membrane; Multi-pass membrane protein Ref.1 Ref.7.
Post-translational modification: The N-terminus is blocked.
Sequence similarities: Belongs to the PA-phosphatase related phosphoesterase family.
Biophysicochemical propertiesKinetic parameters:KM=530 µM for undecaprenyl pyrophosphate Ref.5 Ref.8KM=96 µM for farnesyl pyrophosphateKM=80 µM for diacylglycerol pyrophosphateKM=1700 µM for phosphatidateVmax=2167 nmol/min/mg enzyme with diacylglycerol pyrophosphate as substrateVmax=313 nmol/min/mg enzyme with phosphatidate as substratepH dependence:Optimum pH is 6.5 for DGPP phosphatase activity and 6.5-7.5 for undecaprenyl pyrophosphate phosphatase activity.
Mass spectrometry: Molecular mass is 30151 Da from positions 2 - 254. Determined by MALDI. Reported mass includes mass of a C-terminal His6 tag. Ref.8
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Product Notes
The pgpB pgpb (Catalog #AAA1088620) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-254. The amino acid sequence is listed below: MRSIARRTAV GAALLLVMPV AVWISGWRWQ PGEQSWLLKA AFWVTETVTQ PWGVITHLIL FGWFLWCLRF RIKAAFVLFA ILAAAILVGQ GVKSWIKDKV QEPRPFVIWL EKTHHIPVDE FYTLKRAERG NLVKEQLAEE KNIPQYLRSH WQKETGFAFP SGHTMFAASW ALLAVGLLWP RRRTLTIAIL LVWATGVMGS RLLLGMHWPR DLVVATLISW ALVAVATWLA QRICGPLTPP AEENREIAQR EQES. It is sometimes possible for the material contained within the vial of "Phosphatidylglycerophosphatase B (pgpB), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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