Human Phosphoglucomutase-1 ELISA Kit | PGM1 elisa kit
Human Phosphoglucomutase-1 (PGM1) ELISA Kit
Gene Names
PGM1; CDG1T; GSD14
Reactivity
Human
Synonyms
Phosphoglucomutase-1; Human Phosphoglucomutase-1 (PGM1) ELISA Kit; PGM1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PGM1. No significant cross-reactivity or interference between Human PGM1 and analogues was observed.
Sequence Length
100
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
125 pg/mL - 8000 pg/mL
Sensitivity
83.3 pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for PGM1 elisa kit
Intended Uses: For the quantitative detection of Human Phosphoglucomutase-1 (PGM1) concentration in serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PGM1 in Human serum, plasma, tissue homogenates. An antibody specific for PGM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PGM1 in Human serum, plasma, tissue homogenates. An antibody specific for PGM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,283 Da
NCBI Official Full Name
phosphoglucomutase-1 isoform 2
NCBI Official Synonym Full Names
phosphoglucomutase 1
NCBI Official Symbol
PGM1
NCBI Official Synonym Symbols
CDG1T; GSD14
NCBI Protein Information
phosphoglucomutase-1
UniProt Protein Name
Phosphoglucomutase-1
Protein Family
UniProt Gene Name
PGM1
UniProt Synonym Gene Names
PGM 1
NCBI Description
The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010]
Uniprot Description
This enzyme participates in both the breakdown and synthesis of glucose.
Research Articles on PGM1
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Product Notes
The Human PGM1 pgm1 (Catalog #AAA281798) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281798 ELISA Kit recognizes Human PGM1. It is sometimes possible for the material contained within the vial of "Phosphoglucomutase-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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