General Prostaglandin E2 ELISA Kit | PGE2 elisa kit

Prostaglandin E2 ELISA Kit

Cat. Num. AAA763445

• Gene Names: PTGER4; EP4; EP4R
• Reactivity: General
• Synonyms: Prostaglandin E2; Prostaglandin E2 ELISA Kit; PGE2 (Prostaglandin E2); PGE2 elisa kit

• Reactivity: General
• Specificity: This assay has high sensitivity and excellent specificity for detection of PGE2. No significant cross-reactivity or interference between PGE2 and analogues was observed.
• Sequence Length: 488

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PGE2 elisa kit

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506259
• NCBI GeneID: 5734
• NCBI Accession #: NP_000949.1
• NCBI GenBank Nucleotide #: NM_000958.2
• Molecular Weight: 53,119 Da
• NCBI Official Full Name: prostaglandin E2 receptor EP4 subtype
• NCBI Official Synonym Full Names: prostaglandin E receptor 4
• NCBI Official Symbol: PTGER4
• NCBI Official Synonym Symbols: EP4; EP4R
• NCBI Protein Information: prostaglandin E2 receptor EP4 subtype
• UniProt Protein Name: Prostaglandin E2 receptor EP4 subtype
• Protein Family: Prostaglandin E2 receptor
• UniProt Gene Name: PTGER4
• UniProt Synonym Gene Names: PTGER2; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype
• UniProt Entry Name: PE2R4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses. [provided by RefSeq, Jul 2008]

Uniprot Description

PTGER4: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: cell soma; integral to membrane; nuclear membrane; plasma membrane

Molecular Function: prostaglandin E receptor activity; protein binding

Biological Process: chemokinesis; female pregnancy; G-protein signaling, coupled to cAMP nucleotide second messenger; immune response; JNK cascade; maternal process involved in parturition; negative regulation of circadian sleep/wake cycle, REM sleep; negative regulation of cytokine secretion; negative regulation of inflammatory response; negative regulation of integrin activation; negative regulation of interleukin-1 alpha secretion; negative regulation of nitric-oxide synthase biosynthetic process; negative regulation of peptide secretion; positive regulation of bone resorption; positive regulation of cAMP biosynthetic process; positive regulation of cell adhesion; positive regulation of cell proliferation; positive regulation of circadian sleep/wake cycle, non-REM sleep; positive regulation of cytokine secretion; positive regulation of inflammatory response; positive regulation of interleukin-8 production; positive regulation of osteoblast differentiation; positive regulation of smooth muscle cell migration; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of stress fiber formation; response to amino acid stimulus; response to drug; response to lipopolysaccharide; response to mechanical stimulus; response to progesterone stimulus; T-helper cell differentiation