Phosphoglycerate Mutase 2 Recombinant Protein | PGAM2 recombinant protein

Recombinant Phosphoglycerate Mutase 2, Muscle (PGAM2)

Cat. Num. AAA2033537

• Gene Names: PGAM2; GSD10; PGAMM; PGAM-M
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Phosphoglycerate Mutase 2; Recombinant Phosphoglycerate Mutase 2; Muscle (PGAM2); PGAM-M; BPG-dependent PGAM 2; Muscle-specific phosphoglycerate mutase; Phosphoglycerate mutase isozyme M; PGAM2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MATHRLVMVR HGESTWNQEN RFCGWFDAEL SEKGTEEAKR GAKAIKDAKM EFDICYTSVL KRAIRTLWAI LDGTDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEE QVKIWRRSFD IPPPPMDEKH PYYNSISKER RYAGLKPGEL PTCESLKDTI ARALPFWNEE IVPQIKAGKR VLIAAHGNSL RGIVKHLEGM SDQAIMELNL PTGIPIVYEL NKELKPTKPM QFLGDE
• Sequence Length: 253

• Applicable Applications for PGAM2 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 30.7kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Glu236
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 50593010
• NCBI GeneID: 5224
• NCBI Accession #: NP_000281.2
• NCBI GenBank Nucleotide #: NM_000290.3
• Molecular Weight: 28,766 Da
• NCBI Official Full Name: phosphoglycerate mutase 2
• NCBI Official Synonym Full Names: phosphoglycerate mutase 2
• NCBI Official Symbol: PGAM2
• NCBI Official Synonym Symbols: GSD10; PGAMM; PGAM-M
• NCBI Protein Information: phosphoglycerate mutase 2
• UniProt Protein Name: Phosphoglycerate mutase 2
• Protein Family: Phosphoglycerate mutase
• UniProt Gene Name: PGAM2
• UniProt Synonym Gene Names: PGAMM; PGAM-M
• UniProt Entry Name: PGAM2_HUMAN

NCBI Description

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq, Sep 2009]

Uniprot Description

PGAM2: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). A metabolic disorder characterized by myoglobinuria, increased serum creatine kinase levels, decreased phosphoglycerate mutase activity, myalgia, muscle pain, muscle cramps and excercise intolerance. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 3.1.3.13; Phosphatase (non-protein); Isomerase; EC 5.4.2.11; EC 5.4.2.4

Chromosomal Location of Human Ortholog: 7p13-p12

Cellular Component: cytosol; nucleus

Molecular Function: 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; cofactor binding; phosphoglycerate mutase activity

Biological Process: carbohydrate metabolic process; dephosphorylation; gluconeogenesis; glucose metabolic process; glycolysis; Notch signaling pathway; regulation of pentose-phosphate shunt; response to mercury ion; spermatogenesis; striated muscle contraction

Disease: Glycogen Storage Disease X