Human phosphoglycerate mutase 2 (muscle) ELISA Kit | PGAM2 elisa kit

Human phosphoglycerate mutase 2, PGAM2 ELISA Kit

Cat. Num. AAA703596

• Gene Names: PGAM2; GSD10; PGAMM; PGAM-M
• Reactivity: Human
• Synonyms: phosphoglycerate mutase 2 (muscle); Human phosphoglycerate mutase 2; PGAM2 ELISA Kit; GSD10; MGC88743; PGAM-M; PGAMM; BPG-dependent PGAM 2; phosphoglycerate mutase 2; PGAM2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PGAM2. No significant cross-reactivity or interference between Human PGAM2 and analogues was observed.
• Sequence Length: 253

• Samples: Serum, plasma
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Biosynthesis/Metabolism
• Protein Biological Process 2: glyconeogenesis and glycometabolism
• Protein Biological Process 3: Glycolysis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PGAM2 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PGAM2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PGAM2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PGAM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PGAM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 50593010
• NCBI GeneID: 5224
• NCBI Accession #: NP_000281.2
• NCBI GenBank Nucleotide #: NM_000290.3
• UniProt Accession #: P15259
• Molecular Weight: 28,766 Da
• NCBI Official Full Name: phosphoglycerate mutase 2
• NCBI Official Synonym Full Names: phosphoglycerate mutase 2 (muscle)
• NCBI Official Symbol: PGAM2
• NCBI Official Synonym Symbols: GSD10; PGAMM; PGAM-M
• NCBI Protein Information: phosphoglycerate mutase 2; BPG-dependent PGAM 2; phosphoglycerate mutase isozyme M; muscle-specific phosphoglycerate mutase
• UniProt Protein Name: Phosphoglycerate mutase 2
• Protein Family: Phosphoglycerate mutase
• UniProt Gene Name: PGAM2
• UniProt Synonym Gene Names: PGAMM; PGAM-M
• UniProt Entry Name: PGAM2_HUMAN

NCBI Description

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq, Sep 2009]

Uniprot Description

Function: Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.UniRule annotationHAMAP-Rule:MF_01039

Catalytic activity: 2-phospho-D-glycerate = 3-phospho-D-glycerate.UniRule annotationHAMAP-Rule:MF_01039