Human Pepsin A ELISA Kit | PGA3 elisa kit

Human Pepsin A ELISA Kit

Cat. Num. AAA762223

• Gene Names: PGA5; PGA
• Reactivity: Human
• Synonyms: Pepsin A; Human Pepsin A ELISA Kit; PGA (Pepsinogen A)/PGA3/PGA4/pepsin A/pepsinogen 4; group I (pepsinogen A)/pepsinogen A4/PGA5; PGA3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PGA3. No significant cross-reactivity or interference between PGA3 and analogues was observed.
• Sequence Length: 385

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.56-100ng/ml
• Sensitivity: 0.938ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PGA3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PGA3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PGA3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PGA3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PGA3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PGA3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PGA3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 292658825
• NCBI GeneID: 396892
• NCBI Accession #: NP_999038.2
• NCBI GenBank Nucleotide #: NM_213873.2
• Molecular Weight: 41,262 Da
• NCBI Official Full Name: pepsin A preproprotein
• NCBI Official Symbol: PGA5
• NCBI Official Synonym Symbols: PGA
• NCBI Protein Information: pepsin A
• UniProt Protein Name: Pepsin A
• Protein Family: Pepsin
• UniProt Gene Name: PGA
• UniProt Entry Name: PEPA_PIG

Uniprot Description

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.