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Formate acetyltransferase 1 (pflB) Recombinant Protein | pflB recombinant protein

Recombinant Escherichia coli Formate acetyltransferase 1 (pflB)

Gene Names
pflB; ECK0894; JW0886; pfl
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Formate acetyltransferase 1 (pflB); Recombinant Escherichia coli Formate acetyltransferase 1 (pflB); pflB recombinant protein
Ordering
For Research Use Only!
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
2-760, full length protein
Sequence
SELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFLAGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQTEAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRCRKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTIRLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVSIDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYEASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQFGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVRKTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQSM
Sequence Length
759
Species
Escherichia coli (strain K12)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
85,357 Da
NCBI Official Full Name
formate C-acetyltransferase 1, anaerobic; pyruvate formate-lyase 1
NCBI Official Symbol
pflB
NCBI Official Synonym Symbols
ECK0894; JW0886; pfl
NCBI Protein Information
formate C-acetyltransferase 1, anaerobic; pyruvate formate-lyase 1
UniProt Protein Name
Formate acetyltransferase 1
Protein Family
UniProt Gene Name
pflB
UniProt Synonym Gene Names
pfl

NCBI Description

HT_Cmplx8_Cyt: [More information is available at EcoGene: EG10701].

Uniprot Description

MiscellaneousSeveral mechanisms have been proposed based on complexes formed with substrate analogs. After activation by the glycine radical, the cysteine radical, Cys-420, can abstract hydrogen atoms from the other active site cysteine, Cys-419, and from coenzyme A, and it can also transfer hydrogen atoms to product radicals. The other active site cysteine can attack the central carbonyl of pyruvate and covalently bind the product acetyl group.

Research Articles on pflB

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Product Notes

The pflB pflb (Catalog #AAA1203954) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-760, full length protein. The amino acid sequence is listed below: SELNEKLATA WEGFTKGDWQ NEVNVRDFIQ KNYTPYEGDE SFLAGATEAT TTLWDKVMEG VKLENRTHAP VDFDTAVAST ITSHDAGYIN KQLEKIVGLQ TEAPLKRALI PFGGIKMIEG SCKAYNRELD PMIKKIFTEY RKTHNQGVFD VYTPDILRCR KSGVLTGLPD AYGRGRIIGD YRRVALYGID YLMKDKLAQF TSLQADLENG VNLEQTIRLR EEIAEQHRAL GQMKEMAAKY GYDISGPATN AQEAIQWTYF GYLAAVKSQN GAAMSFGRTS TFLDVYIERD LKAGKITEQE AQEMVDHLVM KLRMVRFLRT PEYDELFSGD PIWATESIGG MGLDGRTLVT KNSFRFLNTL YTMGPSPEPN MTILWSEKLP LNFKKFAAKV SIDTSSLQYE NDDLMRPDFN NDDYAIACCV SPMIVGKQMQ FFGARANLAK TMLYAINGGV DEKLKMQVGP KSEPIKGDVL NYDEVMERMD HFMDWLAKQY ITALNIIHYM HDKYSYEASL MALHDRDVIR TMACGIAGLS VAADSLSAIK YAKVKPIRDE DGLAIDFEIE GEYPQFGNND PRVDDLAVDL VERFMKKIQK LHTYRDAIPT QSVLTITSNV VYGKKTGNTP DGRRAGAPFG PGANPMHGRD QKGAVASLTS VAKLPFAYAK DGISYTFSIV PNALGKDDEV RKTNLAGLMD GYFHHEASIE GGQHLNVNVM NREMLLDAME NPEKYPQLTI RVSGYAVRFN SLTKEQQQDV ITRTFTQSM. It is sometimes possible for the material contained within the vial of "Formate acetyltransferase 1 (pflB), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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