Typical Testing Data/Standard Curve (for reference only)
Rabbit Platelet Factor 4 ELISA Kit | PF4 elisa kit
Rabbit Platelet Factor 4 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This PF4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit PF4. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY PF4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PF4 antibody and an PF4-HRP conjugate. The assay sample and buffer are incubated together with PF4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PF4 concentration since PF4 from samples and PF4-HRP conjugate compete for the anti-PF4 antibody binding site. Since the number of sites is limited, as more sites are occupied by PF4 from the sample, fewer sites are left to bind PF4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PF4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. [provided by RefSeq, Mar 2012]
Uniprot Description
PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Secreted, signal peptide; Apoptosis; Secreted; Motility/polarity/chemotaxis; Chemokine
Chromosomal Location of Human Ortholog: 4q12-q21
Cellular Component: extracellular space; extracellular region
Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity
Biological Process: negative regulation of cytolysis; platelet activation; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; positive regulation of tumor necrosis factor production; negative regulation of megakaryocyte differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; platelet degranulation; negative regulation of MHC class II biosynthetic process; immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; positive regulation of macrophage differentiation; inflammatory response
Research Articles on PF4
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Product Notes
The Rabbit PF4 pf4 (Catalog #AAA723021) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA723021 ELISA Kit recognizes Rabbit PF4. It is sometimes possible for the material contained within the vial of "Platelet Factor 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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