phospho-B-Raf (Ser-446) Peptide

Synonyms

phospho-B-Raf (Ser-446) Peptide; phospho-B-Raf (Ser-446) Peptide peptide

Ordering
Cat. Num. AAA474024 For Research Use Only!

Specificity

The peptide is specifically recognized by anti-B-Raf (Ser-446) antibody (RP2031) in ELISA, and has been shown to block the reactivity of RP2031 during Western blot. In addition, the peptide is recommended for use in blocking RP2031 reactivity in immunocytochemistry.

Form/Format

Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.

Sequence

Peptide Sequence: Phospho-B-Raf (Ser-446) synthetic peptide corresponds to amino acids surrounding serine 446 in human B-Raf. This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299).

Application Notes

ELISA: 50 ng/well
Western Blot: 1:1000

Preparation and Storage

Store at -20 degree C. Stable for 1 year.

Related Product Information for phospho-B-Raf (Ser-446) Peptide peptide

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras- GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf(S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the Nregion and the activation segment. Activation of C-Raf involves phosphorylation at many phosphorylation sites including Ser-338, Tyr -341, and multiple catalytic domain sites. In B-Raf, multiple phosphorylation sites have been identified, but their specific roles are uncertain. Phosphorylation of Ser-446 may prime B-Raf for activation, and Ser-446 and/or Ser-447 phosphorylation may be critical for B-Raf biological activity during PC12 differentiation. Ser-579 is required for growth factor activation and kinase activity. Thus, multiple sites of phosphorylation within Rafs may be important for regulation of their activity.

References

• Mason, C.S. et al. (1999) EMBOJ 18(8):2137.
• Wilhelm, S.M. et al. (2004) Cancer Res 64:7099.
• Karbowniczek, M. et al. (2006) J Biol Chem 281(35):25447.
• Brummer, T. et al. (2006) Oncogene 25(47):6262.

NCBI and Uniprot Product Information

NCBI GI #

7271193

NCBI Official Full Name

B-Raf protein

Similar Products

Product Notes

The phospho-B-Raf (Ser-446) Peptide (Catalog #AAA474024) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. ELISA: 50 ng/well Western Blot: 1:1000. Researchers should empirically determine the suitability of the phospho-B-Raf (Ser-446) Peptide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Peptide Sequence: Phospho-B- Raf (Ser-446) synthetic peptide correspond s to amino acids surroundin g serine 446 in human B-Raf. This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299). It is sometimes possible for the material contained within the vial of "phospho-B-Raf (Ser-446) Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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phospho-B-Raf (Ser-446) Peptide