Neuropilin-1 (a1 CUB Domain) Peptide | NRP1 peptide

Neuropilin-1 (a1 CUB Domain) Peptide

Cat. Num. AAA474014

• Gene Names: NRP1; NP1; NRP; BDCA4; CD304; VEGF165R; DKFZp781F1414; DKFZp686A03134
• Synonyms: Neuropilin-1 (a1 CUB Domain) Peptide; NRP1 peptide

• Specificity: The peptide is specifically recognized by anti-Neuropilin 1 (a1 CUB domain) antibody (NP2111) in ELISA, and has been shown to block the reactivity of NP2111 in Western blot and immunocytochemistry applications.
• Form/Format: Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
• Sequence: Peptide Sequence: Neuropilin-1 synthetic peptide corresponds to amino acids within the a1 CUB domain of human neuropilin-1. This sequence is highly conserved in rat and mouse neuropilin-1, and has low homology to neuropilin-2.
• Sequence Length: 923

• Application Notes: Blocking: 1:1,000; ELISA: 50 ng/well
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Related Product Information for NRP1 peptide

Neuropilins are transmembrane proteins that contain two CUB domains (a1 and a2), two coagulation factor-like domains (b1 and b2), and a MAM domain in the extracellular region. These proteins have short cytoplasmic domains that include a PDZ-binding motif. The neuropilin (NRP) family includes NRP-1, NRP-2A, and NRP-2B. NRP-1 has been implicated as a receptor involved in axon guidance and VEGF signaling. NRP-1 mediates activation of intracellular signaling pathways through interaction with its co-receptors, Plexin-A1 and VEGFRs. The expression of NRP-1, along with the co-receptor Plexin-A1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.

References

• He, Z. & Tessier-Lavigne, M. (1997) Cell 90:739.
• Nakamura, F. & Goshima, Y. (2002) Adv Exp Med Biol. 515:55.
• Takahashi, T. et al. (1999) Cell 99:59.
• Saban, M.R. et al. (2008) Am J Phys Renal Phys 295(1):F60 (ICC: H. Urothelium)

NCBI and Uniprot Product Information

• NCBI GI #: 182508169
• NCBI GeneID: 8829
• NCBI Accession #: NP_003864.4
• NCBI GenBank Nucleotide #: NM_003873.5
• UniProt Accession #: O14786; O60461; Q5T7F2; Q5T7F3; Q86T59; Q96IH5; B0LPG9; Q59F20; Q5JWQ0; Q5JWQ1; Q5JWQ2; Q5JWQ4; Q5JWQ5; Q5JWQ6; Q5T7F0; Q5T7F1
• Molecular Weight: 102976 Da
• NCBI Official Full Name: neuropilin-1 isoform a
• NCBI Official Synonym Full Names: neuropilin 1
• NCBI Official Symbol: NRP1
• NCBI Official Synonym Symbols: NP1; NRP; BDCA4; CD304; VEGF165R; DKFZp781F1414; DKFZp686A03134
• NCBI Protein Information: neuropilin-1; OTTHUMP00000020818; OTTHUMP00000020819; OTTHUMP00000020820; OTTHUMP00000020821; OTTHUMP00000020822; transmembrane receptor; vascular endothelial cell growth factor 165 receptor
• UniProt Protein Name: Neuropilin-1
• Protein Family: Neuropilin
• UniProt Gene Name: NRP1
• UniProt Synonym Gene Names: NRP; VEGF165R
• UniProt Entry Name: NRP1_HUMAN

NCBI Description

NRP1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF; MIM 192240) and semaphorin (see SEMA3A; MIM 603961) family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.[supplied by OMIM]

Uniprot Description

Function: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.

Subunit structure: Homodimer, and heterodimer with NRP2. Binds PLXNB1. Ref.17

Subcellular location: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Tissue specificity: The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.

Domain: The tandem CUB domains mediate binding to semaphorin, while the tandem F5/8 domains are responsible for heparin and VEGF binding.

Sequence similarities: Belongs to the neuropilin family.Contains 2 CUB domains.Contains 2 F5/8 type C domains.Contains 1 MAM domain.