PELI2 recombinant protein

PELI2, 1-420aa, Human, His tag, E Coli

Cat. Num. AAA205987

• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: PELI2; 1-420aa; Human; His tag; E Coli; E3 ubiquitin-protein ligase pellino homolog 2; PELI2 - pellino E3; Pellino-2; PELI2 recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMFSPGQE EHCAPNKEPV KYGELVVLGY NGALPNGDRG RRKSRFALYK RPKANGVKPS TVHVISTPQA SKAISCKGQH SISYTLSRNQ TVVVEYTHDK DTDMFQVGRS TESPIDFVVT DTISGSQNTD EAQITQSTIS RFACRIVCDR NEPYTARIFA AGFDSSKNIF LGEKAAKWKN PDGHMDGLTT NGVLVMHPRG GFTEESQPGV WREISVCGDV YTLRETRSAQ QRGKLVESET NVLQDGSLID LCGATLLWRT ADGLFHTPTQ KHIEALRQEI NAARPQCPVG LNTLAFPSIN RKEVVEEKQP WAYLSCGHVH GYHNWGHRSD TEANERECPM CRTVGPYVPL WLGCEAGFYV DAGPPTHAFT PCGHVCSEKS AKYWSQIPLP HGTHAFHAAC PFCATQLVGE QNCIKLIFQG PID
• Sequence Length: 420

• Applicable Applications for PELI2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PELI2 recombinant protein

PELI2 is an E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. This protein is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. It mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. PELI2 may be important for LPS- and IL1B-induced MAP3K7-dependent, but not MAP3K3-dependent, NF-kappa-B activation. It can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1. Recombinant human PELI2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PELI2 recombinant protein

Signal Transduction

References

Jensen L.E., et al (2003). FEBS Lett. 545:199-202; Strelow A., et al (2003). FEBS Lett. 547:157-161

NCBI and Uniprot Product Information

• NCBI GI #: 10864063
• NCBI GeneID: 57161
• NCBI Accession #: NP_067078
• NCBI GenBank Nucleotide #: NM_021255.2
• UniProt Accession #: Q9HAT8; B2RDY5
• Molecular Weight: 48.8 kDa (443aa) confirmed by MALDI-TOF
• NCBI Official Full Name: E3 ubiquitin-protein ligase pellino homolog 2
• NCBI Official Synonym Full Names: pellino E3 ubiquitin protein ligase family member 2
• NCBI Official Symbol: PELI2
• NCBI Protein Information: E3 ubiquitin-protein ligase pellino homolog 2
• UniProt Protein Name: E3 ubiquitin-protein ligase pellino homolog 2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: PELI2
• UniProt Synonym Gene Names: Pellino-2

Uniprot Description

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. May be important for LPS- and IL1B-induced MAP3K7-dependent, but not MAP3K3-dependent, NF-kappa-B activation. Can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1.