Rat pyruvate dehydrogenase kinase, isozyme 2 ELISA Kit | PDK2 elisa kit

Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial, PDK2 ELISA Kit

Cat. Num. AAA9330601

• Reactivity: Rat
• Synonyms: pyruvate dehydrogenase kinase; isozyme 2; Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2; mitochondrial; PDK2 ELISA Kit; mitochondrial (PDK2) ELISA kit; PDHK2; pyruvate dehydrogenase kinase 2; isoenzyme 2; pyruvate dehydrogenase; lipoamide; kinase isozyme 2; PDK2 elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Mouse PDK2 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25ng/ml-8ng/ml.
• Sensitivity: 0.1ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated PDK2 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse PDK2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PDK2 and PDK2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PDK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for PDK2 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PDK2 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse PDK2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PDK2 and PDK2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PDK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13540705
• NCBI GeneID: 81530
• NCBI Accession #: NP_110499.1
• NCBI GenBank Nucleotide #: NM_030872.1
• UniProt Accession #: Q64536
• Molecular Weight: 46,106 Da
• NCBI Official Full Name: pyruvate dehydrogenase kinase, isozyme 2
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase, isozyme 2
• NCBI Official Symbol: Pdk2
• NCBI Protein Information: pyruvate dehydrogenase kinase, isozyme 2; PDK P45; PDH kinase 2; pyruvate dehydrogenase 2; pyruvate dehydrogenase kinase, isoenzyme 2; pyruvate dehydrogenase kinase 2 subunit p45 (PDK2); [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
• UniProt Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
• UniProt Gene Name: Pdk2
• UniProt Synonym Gene Names: PDH kinase 2
• UniProt Entry Name: PDK2_RAT

NCBI Description

catalyzes the phosphorylation and inactivation of the pyruvate dehydrogenase complex; plays a role in the regulation of glucose oxidation [RGD, Feb 2006]

Uniprot Description

PDHK2: an atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK2 is expressed in all tissues. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: Kinase, protein; Protein kinase, atypical; Mitochondrial; EC 2.7.11.2; ATYPICAL group; PDHK family; PDHK subfamily

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; pyruvate dehydrogenase complex; mitochondrial pyruvate dehydrogenase complex

Molecular Function: protein serine/threonine kinase activity; protein homodimerization activity; protein heterodimerization activity; protein complex binding; pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding

Biological Process: peptidyl-serine phosphorylation; insulin receptor signaling pathway; glucose metabolic process; regulation of pH; cellular response to nutrient; regulation of acetyl-CoA biosynthetic process from pyruvate; regulation of gluconeogenesis; glucose homeostasis; protein amino acid phosphorylation